The Screening of High Virulence Bacillus thuringiensis Strains to Chilo suppressalis (Walker)(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: The Screening of High Virulence Bacillus thuringiensis Strains to Chilo suppressalis (Walker)

Author(s):: ZHANG Xin-lin¹; YU Hong-chun¹; FANG Dan-dan¹; XIANG Dan-dan¹; XU Zhen-nan¹; XU Guo-qing²; 1.School of Agriculture,Northeast Agricultural University,Harbin,Heilongjiang 150030;
2.Plant Protection Institute,Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161

Keywords:: Chilo suppressalis (Walker); Bacillus thuringiensis; strains; bioassay; virulence; screening

Abstract:: Taking Chilo suppressalis as material,using high virulence Bt strains,the effect of 30 Bt strains on the virulence test of 1~2 instar ?Chilo suppressalis larvaes were studied,then the effect of 5 high virulence Bt strains that screened out on virulence of 2~3 instar Chilo supprealis larvaes were studied,to screen out suitable high virulence Bt strain to prevention Chilo suppressalis in Heilongjiang province.The results showed that the corrected mortality of Chilo suppressalis was 90% treated by Bt14 bacteria liquid after 168 h.And then its toxicity regression equations were:y=0.6121x+0.8789，R²=0.9989.Its LC₅₀?was 5.4×10⁶ spores/mL,which was significant difference from other Bt stains.The results suggested that Bt14 could have a good prospect for controlling Chilo suppressalis.

［1］徐艳博,王孟伦,韩兰芝,等.人工饲料、转Bt水稻及其亲本稻苗饲养下二化螟种群适合度的比较研究［J］.应用昆虫学报,2012,49(6):1535-1540.

［2］姚英娟,徐雪亮,徐荣仔,等.不同药剂混配组合对二化螟的防治效果［J］.华中农业大学学报,2013,32(4):43-47.

［3］耿欢欢,李红霞,于洪春,等.黑龙江省二化螟虫口密度与粳稻产量损失关系及经济阈值的研究［J］.东北农业大学学报,2012,43(1):43-47.

［4］彭丽年,张伟,蒋凡,等.氯虫苯甲酰胺对二化螟的防效简报［J］.中国植保导刊,2011,31(6):42-43.

［5］吕锐玲,周强,涂军明,等.水稻二化螟的发生与防治［J］.现代农业科技,2011(14):188-192.

［6］张帅,李永平,邵振润,等.水稻二化螟抗药性监测及防控对策［J］.植物保护,2011,37(2):141-144.

［7］姚英娟,徐雪亮,曾水根,等.二化螟对不同药剂的敏感度研究［J］.植物保护,2013,39(6):95-99.

［8］朱红军,徐庚鸿,赵德明,等.水稻病虫害绿色防控技术研究与集成示范［J］.安徽农业科学,2013,41(7):2924-2925.

［9］郭荣,韩梅,束放.减少稻田用药的病虫害绿色防控策略与措施［J］.中国植保导刊,2013,33(10):38-41.

［10］郑和斌.水稻病虫绿色防控技术的应用及效益［J］.中国植保导刊,2012,32(7):58-60.

［11］彭丽年,蒋凡,徐翔,等.甜核?苏云菌对水稻二化螟的防效［J］.植物保护,2011,37(3):170-172.

［12］韩新才.提高苏云金杆菌防治效果技术的研究［J］.安徽农业科学,2006,34(8):1614-1616.

Last Update: 2014-09-26