Optimization of Culture Conditions in vitro Dendranthema indicum var.aromaticum Buds of Orthogonal Test(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Optimization of Culture Conditions in vitro Dendranthema indicum var.aromaticum Buds of Orthogonal Test

Author(s):: LEI Xiao-fei¹; YANG Xue-ling²; 1.Editorial Office of Journal,Wuhan Bioengineering Institute,Wuhan,Hubei 430415；
2.Scientific Research Division,Wuhan Bioengineering Institute,Wuhan,Hubei 430415

Keywords:: Dendranthema indicum var.aromaticum; tissue culture; rapid propagation technique; orthogonal test

Abstract:: Taking buds of Dendranthema indicum var.aromaticum introduced from Shennongjia of Hubei as the explants,the sterilizing effect was studied through different disinfectors and methods,by hormone combination test on sprout medium and rooting medium,rooting hormone ratio were studied to selected the best medium for rapid propagation of Dendranthema indicum var.aromaticum were studied.The results showed that the suitable disinfectant was 75% Alcohol 30 s+0.1% HgCl₂ 2 min+2% NaClO₃ 8 min；the suitable sprout medium was MS+0.7 mg/L 6-BA+0.5 mg/L KT+0.3 mg/L NAA;the suitable rooting medium was 1/2 MS+0.5 mg/L NAA.

［1］谢超,黄龙,肜霖,等.正交试验优选“神农”香菊挥发油提取条件［J］.湖北农业科学,2011,50(21):4451-4453.

［2］刘启去,张红旗,贾卫疆.湖北新资源植物“神农”香菊的地理分布、生态习性与蕴藏量的调查研究［J］.武汉植物学研究,1983,1(2):239-245.

［3］卢金清,万威,徐玉婷,等.“神农”香菊化学成分研究［J］.中药材,2009,32(1):53-55.

［4］田耀平,卢金清.“神农”香菊的研究进展［J］.时珍国医国药,2011,22(2):468.

［5］何淼,董春艳,冯博,等.“神农”香菊组织培养和植株再生［J］.东北林业大学学报,2010,38(7):64-66.

［6］孙明,张启翔.“神农”香菊花蕾的组织培养［J］.植物生理学通讯,2005,41(3):348.

［7］张福生,郭顺星.SPSS正交设计在福建金线莲组织培养中的应用［J］.中国中药杂志,2009,34(20):2581-2595.

Last Update: 2014-09-26