Study on Isolation of Full-length cDNA of HCD from Leaf of Prunus salicina and Its Expression(PDF)

¡¶±±·½Ô°ÒÕ¡·[ISSN:1001-0009/CN:23-1247/S]

Issue:

2014Äê16ÆÚ

Page:

100-104,105

Research Field:

Publishing date:

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Title:

Study on Isolation of Full-length cDNA of HCD from Leaf of Prunus salicina and Its Expression

Author(s):

ZHAO Li¹; 2; WANG Yu-zhen¹; 2; LV Shi-heng¹; PAN Dong-ming¹; 2; JIANG Cui-cui³; CHEN Gui-xin¹; 2

1.College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002;

2.Institute of Storage Science and Technology of Horticultural Products,College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002£»

3.Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013

Keywords:

Prunus salicina; ¦Â-hydroxyacylCoA dehydratase; full-length cDNA; expression analysis

PACS:

Q943.2

DOI:

-

Abstract:

Taking five different growth period of Prunus salicina¡¯s leaves as material,a clone named PsHCD was separated from cDNA library prepared of five different growth and development periods of leaf in the Prunus salicina.The biological information of PsHCD was analyzed and gene expression levels in five different growth periods of Prunus salicina¡¯s leaves were tested using Real-time PCR.The results showed that the gene cDNA open reading frame coding regions for the 322^thnucleotide to 987^th,encoding a 221 amino acid proteins,with 321 bp in the 5¡¯UTR and 156 bp in the 3¡¯UTR.Using TMHMM server software to analysis,the gene encoding the protein had four transmembrane domains.PsHCD was localized in endoplasmic reticulum,microbodies and lysosomes by PSORT ¢ò Prediction software.The results of Real-time PCR showed that the PsHCD gene was gradually increasing with development of leaves,reached to maximum at stage of unfold leaves,was stable during spire to climax leaves,then decreased,reached to minimum at stage of old leaves.

References:

£Û1£ÝKunst L,Samuels L.Plant cuticles shine:advances in wax biosynthesis and export£ÛJ£Ý.Current Opinionin Plant Biology,2009,12(6):721-727.

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