|Table of Contents|

Callus Induction and Antibrowning in Subculture of Sophora alopecuroides L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年16期
Page:
96-99
Research Field:
Publishing date:

Info

Title:
Callus Induction and Antibrowning in Subculture of Sophora alopecuroides L.
Author(s):
CHEN Ya-ping; GAO Yuan; GU Pei-wen
Agricultural College,Ningxia University,Yinchuan,Ningxia 750021
Keywords:
Sophora alopecuroides L.; callus; subculture; antibrowning
PACS:
q946.8
DOI:
-
Abstract:
Taking Sophora alopecuroides as material,the effect of explants type,hormone levels,casein hydrolysate on the growth of callus were measured by tissue culture method.Meanwhile many measures were carried out to prevent browning.The results showed that the cotyledon was the optimum explants of callus induction,the best medium of callus induction was MS+2,4-D 0.5 mg/L+NAA 0.5 mg/L+6-BA 1.0 mg/L;and the best subculture medium was MS+2,4-D 1.0 mg/L+6-BA 4.0 mg/L+NAA 1.0 mg/L+CH 1 000 mg/L;the best proposal was frequent subculture with 3% sucrose.

References:

［1］祁燕蓉,何生虎,史光亮.苦豆子的研究进展［J］.甘肃畜牧兽医,2008,38(6):36-38.

［2］曹有龙,李晓莺,罗青,等.苦豆子的组织培养及植株再生的研究［J］.广西植物,2010,30(1):102-105.
［3］张守润,纪瑛,蔺海明.施氮对苦豆子生物性状和生物量积累动态的响应［J］.草业科学,2008,25(3):37-42.
［4］杨春霞,黄丽莉,章挺,等.苦豆子愈伤组织培养及其药用成分含量测定［J］.中药材,2011,27(18):153-157.
［5］王立强,杨军,王光碧,等.苦豆子愈伤组织的诱导与继代培养的去褐化研究［J］.西华师范大学学报(自然科学版),2008,29(4):421-430.
［6］Homare T.Paclitaxel Production by PlantCellCulture Technology［J］.Adv Biochem Engin/Biotechnol,2004(7):1-23.
［7］王亚琴,朱媛.杜仲细胞悬浮培养桃叶珊瑚苷的研究［J］.广西植物,2007,27(2):236-239.
［8］岳才军,何彦平.人参胚愈伤组织诱导和悬浮培养的研究［J］.安徽农业科学,2010(14):7193-7194.
［9］李晓莺,曹有龙,贝盏临,等,苦豆子组织培养初步研究［J］.甘肃农业科技,2004(12):12-14.
［10］古力娜?沙比儿,吴韬,阿吉艾克拜尔?艾萨,等.苦豆子HPLC色谱指纹图研究［J］.中药材,2008,1(31):38-41.
［11］Nguyen T P T,Yukio O.Callus induclion and plantlet regeneration in ornamental A locasia micholitzana［J］.Plant Cell,Tissue snd Organ Culture,2003,73:285-289.
［12］田志宏,李小丽,严寒.不同生物调节剂对马蹄金愈伤组织诱导的影响［J］.广西植物，2004,24(3):253-258.

Memo

Memo:
-
Last Update: 2014-09-26