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Study on Pollen Viability and Storage Characters of Chaenomeles speciosa(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年16期
Page:
81-85
Research Field:
Publishing date:

Info

Title:
Study on Pollen Viability and Storage Characters of Chaenomeles speciosa
Author(s):
MA Shi-yu1; ZHOU Lan-ying1; PU Guang-lan1; LAI Teng-yue1; CAI Li-juan1; WANG Bo1; 2
1.College of Forestry,Sichuan Agricultural University,Ya’an,Sichuan 625014；
2.Department of Architectural Engineering,Sichuan Engineering Technical College,Deyang,Sichuan 618000
Keywords:
Chaenomeles speciosa（Sweet）Nakai; pollen; viability; storage; germinationo germinate easily.If the concentration exceeded optimum capacity; germination rate of pollen would be restrained.Moreover; high concent
PACS:
s661.4
DOI:
-
Abstract:
Taking young bloom Chaenomeles speciosa as experimental materials，four coloring agents (Methylene Blue,TTC,I2-KI and alpha-naphthol benzidine) were used to measure pollen viability.Storage characters under three different temperature conditions (-20,4 and 25℃) and germination percentage under different concentrations of sucrose,boric acid and temperature were studied respectively.The results showed that pollen viability measured by different coloring agents showed significantly differences.Methylene Blue(84.94%) was the best choices for rapid determination,while the worst was I2-KI(44.62%).Storage temperatures had remarkable effects on pollen viability,that was the lower the temperature,the longer the storage time.The storage time of pollen viability percentage under 50% were 3 days(25℃),10 days(4℃) and 7 days(-25℃),the optimum temperature for pollen storage was 4℃.Within certain limits,sucrose,boric acid and temperature could promote pollen tration of boric acid gave a severe inhibition on germination by contrast with low boric acid concentration.The optimal sucrose,boric acid concentration and temperature were 10%，0.03%，20℃ respectively during single factor experiment.The optimal portfolio of solid medium verified by orthogonal test was 8%(sucrose)+0.03%(boric acid)+20℃(temperature).

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Last Update: 2014-09-26