Comparison of Separation Method and Performance Comparison Among Different High Quality Stock Culture of Cordyceps militaris(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Comparison of Separation Method and Performance Comparison Among Different High Quality Stock Culture of Cordyceps militaris

Author(s):: FANG Hua-zhou; Institute of Biological Engineering,Jingchu Science and Technology College,Jingmen,Hubei 448000

Keywords:: Cordyceps militaris; tissue separation; spore cultivation; strain; cultivation characteristics; yield

Abstract:: Taking the Cordyceps militaris quality maternal as female parent，the progeny strains prepared from tissue separation and spore cultivation as test group and the original stock culture as control group，the rejuvenation methods of Cordyceps militaris were determined by comparing the test results of strain characteristics and cultivation.The results showed that the tissue isolation fine strains rate of 85%,was 30% higher than the spore culture method,spore culture method could produce a certain proportion of better strains.It proved that tissue isolation method was more suitable for the genetic and maternal character,spore culture method was especially suitable for screening better strains,both as a fine strain of Cordyceps militaris rejuvenation method.

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Last Update: 2014-09-21