Optimization of Media for Inducing Ovule Culture of ?Platycodon grandiflorum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Optimization of Media for Inducing Ovule Culture of ?Platycodon grandiflorum

Author(s):: XU Fang-fang¹; ZHAO Li-li¹; XUN Yang²; YANG Li¹; JIN Jiang-shan¹; YAN Yi-zi¹; 1.Agricultural College,Yanbian University,Yanji,Jilin 133002；
2.Seed Management Station of Yanbian State,Yanji,Jilin 133001

Keywords:: Platycodon grandiflorum; ovule; tissue culture; induction rate; fresh calli weight

Abstract:: Taking unfertilized ovules of Platycodon grandiflorum as materials,the effect of different combinations of auxin and cytokinin on the callus induction and fresh weight were studied and compared,in order to screen out the best induction medium.The results showed that two optimal media for inducing unfertilized ovules to form calli were selected,the first optimal medium was N6+1.0 mg/L 2,4-D+0.1 mg/L KT,the induction rate was 100% and the average fresh weight of callus was large； the second optimal medium was N6+0.5 mg/L NAA+0.1 mg/L KT,the induction rate was 90% and the average fresh weight of callus was the largest.

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Last Update: 2014-09-21