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Establishment and Optimization of ISSR-PCR Reaction System for Polyscias(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年15期
Page:
101-105
Research Field:
Publishing date:

Info

Title:
Establishment and Optimization of ISSR-PCR Reaction System for Polyscias
Author(s):
ZHANG Guo-wu1; HUANG Tao2; WU Nan-sheng2; ZHANG Pei-jian1; LIU Xue-feng1
1.Eucalypt Research Centre,State Forestry Administration,Zhanjiang,Guangdong 524022；
2.College of Landscape and Art,Jiangxi Agricultural University,Nanchang,Jiangxi 330045
Keywords:
.Polyscias; ISSR-PCR reaction; gradient PCR; annealing temperature
PACS:
S682.36
DOI:
-
Abstract:
Use leaves of Polyscias as materials,the effect of the five main reaction elements (Mg2+,Taq DNA polymerase,dNTP,DNA template and primer) on Polyscias ISSR-PCR were all optimized by combining single factor experiments and orthogonal test method.The results showed that the best ISSRPCR reaction system of Polyscias was 25 μL,reaction system concention：the DNA template 15.00 ng,Mg2+3.0 mmol/L,Taq DNA polymerase 0.5 U,primer 0.6 μmol/L,dNTP 0.20 mmol/L.The optimal annealing temperature of ISSR-PCR was 47℃ by gradient PCR.This system could provide theoretical foundation for carrot research on genetic diversity analysis,genetic relationship,etc

References:

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Last Update: 2014-09-21