|Table of Contents|

Distribution and Polymorphism Characteristics of SSR Loci in Melon Genome(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年15期
Page:
93-96
Research Field:
Publishing date:

Info

Title:
Distribution and Polymorphism Characteristics of SSR Loci in Melon Genome
Author(s):
GAO Xing-wang1WANG Xian-lei2YANG Ya-ting2ZHANG Zi-qiang1LU Hao2LI Guan2
1.College of Resources and Environmental Science,Xinjiang University,Urumqi,Xinjiang 830046;
2.College of Life Science and Technology,Xinjiang University,Urumqi,Xinjiang 830046
Keywords:
melongenomeSSRdistributionpolymorphism
PACS:
S652
DOI:
-
Abstract:
Taking three kinds of melon including ‘BM7’、‘Jiashi’ and ‘Y8’ as materials,three fragments of the melon genome sequence were randomly selected for investigation of distribution characteristics of SSR in melon genome;polyacrylamide gel electrophoresis was used to test the polymorphism of self designed SSR primers.The results showed that the mean distribution density of SSR was one SSR loci per 1.9 kb.Motif rich in A and T appears much higher than motif rich in C and G,motif comprised all by C and/or G was not found.The largest proportion of SSR loci was the dinucleotide,trinucleotide and tetranucleotide repeat motifs,and the total ratio was 83.3%.The proportion of pentanucleotide and hexanucleotide was low,with the ratio of 16.7%.The average lengths of dinucleotide to hexanucleotide repeat motifs were 21,15,14,16,20 bp respectively.The PCR products can be amplified by 81 of total 82 designed self designed SSR primers successfully,and the maximal polymorphism was 48.1%.The results showed that the distribution of SSR in melon genome was relative stable,and it is helpful for future work in SSR and ISSR.

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Last Update: 2014-09-20