Study on Plant Regeneration from Microspore-derived Embryos in Cabbage(Brassica oleraceavar.capitata)(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2014年01期

Page:

97-101

Research Field:

Publishing date:

Info

Title:

Study on Plant Regeneration from Microspore-derived Embryos in Cabbage(Brassica oleraceavar.capitata)

Author(s):

GAO Hai-na¹; WANG Zhao-yang¹; ZHANG En-hui²

1.Agricultural Science Research Institute of Ankang,Ankang,Shaanxi 725000;

2.College of Horticulture,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100

Keywords:

cabbage; microspore; cotyledon type embryoids; plant regeneration

PACS:

S 635

DOI:

-

Abstract:

Taking 3 superior cabbage varieties as test materials,isolated microspores culture and embryoid plant let regeneration technology were studied,the effects of different genotypes,different type of embryoids,different size of shoots,uniconazole on embryoids seedling induction were discussed.The results showed that cotyledon embryoids were easily form to single plants,and torpedo embryoids and ball-type embryoids were easily form to multiple shoots in all different genotypes.The rate of single plants and multiple shoots were different in different genotypes.The size of adventitious shoots was larger than 2 cm,the growth rate showed slow to fast after transferred to rooting medium,and the roots were fine and longer,The size of adventitious shoots shorter than 2 cm,the growth rate showed fast to slow after transferred to rooting medium,and the roots were healthy and strong.Add 0.50 mg/L uniconazole MS+0.3 mg/L NAA regenerated plantlets rooting medium up to 95.6% survival rate of regenerated shoots thick stems and roots.

References:

[1] Fletcher R,Coventry,Kott L S.Double haploid technology for winter Brassica napus [D].Canada University of Guelph,1998：42.

[2] 杨丽梅，方智远，刘玉梅,等.“十一五”我国甘蓝遗传育种研究进展[J].中国蔬菜，2011(2)：1-10.

[3] Kuginuki Y,Yoshikawa H,Hirai M.Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot-resistant cultivars of Chinese cabbage (Brassica rapaL.ssp.pekinensis)[J].European Journal of Plant Pathology,1999：105(4):327-332

[4] Chuong P V,Beversdorf W D,Powell A D,et al.The use of haploid protoplast fusion to combine cytoplasmic atrazine resistance and cytoplasmic male sterility in Brassicanapus[J].Plant CelTissue and Organ Culture,1988,12(2):181-184

[5] Caredda S,Doncoeur C,Devaux P,et al.Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley (Hordeum vulgareL.)[J].Sexual Plant Reproduction,2000,13(2)：95-104.

[6] 马文卿,李青,刘燕.大花蕙兰试管苗增殖过程中的褐化研究[J].中国农学通报,2010,26(7):186-190.

[7] 崔广荣,刘云兵,谷业理.琼脂浓度、pH值及接种苗大小对文心兰试管苗增殖的影响[J].生物学杂志,2004,21(6):29-31.

[8] 王会.红实美草莓试管苗瓶外生根的研究[J].长江蔬菜,2008(22):44-47.

[9] 马勇斌,张恩慧,李殿荣,等.利用甘蓝游离小孢子不定芽叶片再生DH植株技术研究[J].西北农林科技大学学报(自然科学版),2011(4):111-116.

[10]许念芳.诱导甘蓝小孢子高出胚率和成苗技术研究[D].杨凌:西北农林科技大学，2010:23.

[11]周祖富,艾素云,杨美纯.烯效唑对罗汉果试管苗生长的影响[J].福建果树,2005(1):13-15.

[12]葛云侠,陈凤玉.S3307对番茄插枝生根的作用[J].植物生理学通讯,2001,37(1):15-17.

Memo

Memo:

-

Common functions