|Table of Contents|

Cloning and Tissues Expression Analysis of Soluble Acid Invertase Gene from Lycium barbarum.L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年01期
Page:
86-90
Research Field:
Publishing date:

Info

Title:
Cloning and Tissues Expression Analysis of Soluble Acid Invertase Gene from Lycium barbarum.L.
Author(s):
WANG Li-juan; ZHAO Hui; WANG Yan-cai; DING Xiang-zhen; MA Jian-ming
College of Life Science,Ningxia University,Yinchuan,Ningxia 750021
Keywords:
-
PACS:
R 282.4
DOI:
-
Abstract:
Using Lycium barbarum. L.‘Ningqi No.1’ as material,cloning and tissue expression analysis of SAIgenes of Lycium barbarum.L. was studied,and using MEGA 5.0 software to SAIgenes for phylogenetic tree was analyzed.The results showed that by RT-PCR and RACE methods a SAI gene named as Lb SAI (GenBank number：KC776575) was identified from Lycium barbarumL.the full length cDNA of Lb SAI was 2 193 bp;the amino acid sequences of Lb SAIwere 68%～100% identical to the sequences of potato,tomato,pear etc.The putative protein encoded byLb SAIwas belonged to cell wall invertase.Real-time PCR analysis showed that the Lb SAIgene was the highest expressed in flower,the expression level reached the lowest level in root.

References:

[1] 白寿宁.宁夏枸杞研究[M].银川：宁夏人民出版社，1999:29-30．

[2] Schaffer A A,Aloni B,Fogelman E.Sucrose metabolism and accumulation in developing fruit ofCucumis[J].Phytochem,1987(26)：1883-1887.

[3] 王永章,张大鹏.“红富士”苹果果实蔗糖代谢与酸性转化酶和蔗糖合成酶关系的研究[J].园艺学报,2001,28(3):259-261.

[4] Hubbard N L,Parr D M,Huber S C.Sucrose-phosphate synthase and other sucrose metabolizing enzymes in fruit of various species[J].Physiol Plant,1991,82:191-196.

[5] Winter H,Huber S C.Regulation of sucrose and regulation of activity of key enzymes[J].Crit Rev Plant Sci,2000,19(1)：31-67.

[6] 郑国琦,罗霄,买立芸,等.不同品种宁夏枸杞果实糖积累差异的生理原因[J].农业科学研究,2009,30(1):1-4.

[7] Haouazine T N,Tymowska L Z,Takvorian A,et al.Characterization of two members of theArabidopsis thalianagene family,Atbfruct3 and Atbfruct4,coding for vacuolar invertases[J].Gene,1997,197:239-251.

[8] Lorenz K,Lienhard S,Sturm A.Structural organization and differential expression of carrot b-fructofuranosidase genes：identification of a gene coding for a flower bud_specific isozyme[J].Plant Mol Biol,1995(28):189-194.

[9] Mercier R W,Gogarten J P.A second cell_wall acid invertase gene inArabidopsis thaliana[J].Plant Physiol,1995,107：659-660.

[10]Kim J Y,Mahe A,Sylvain G,et al.Characterization of two members of the maize gene family,Incw3 and Incw4,encoding cell_wall invertases[J].Gene,2000,245:89-102.

[11]Davies C,Robinson S P.Sugar accumulation in grape berries,cloning of two putative vacuolar invertase cDNAs and their expression in grapeving tissues [J].Plant Physiol,1996,111:275-283.

[12]Xu J,Avigne W T,Mccarty D R.A similar dichotomy of sugar modulation and developmental expression affects both paths of sucrose metabolism.Evidence from a maize invertase gene family[J].Plant Cell,1996(8):1202-1220.

[13]Sturm A.Molecular characterization and functional analysis of sucrose-cleaving enzymes in carrot (DaucuscarotaL.)[J].J Exp Bot,1996,47:1187-1192.

[14]Tymowska-Lalanne Z,Kreis M.Expression of theArabidopsis thalianainvertase gene family[J].Planta,1998b,207：259-265.

[15]Ranwala A P,Baird W V,Miller W E.Organ specific localization and molecular properties of three soluble invertase fromLilium longiflorumflower buds[J].Physiologia Plantarum,1998,103：551-559.

[16]Lorenz K,Lienhard S,Strum A.Structural organization and differential expression of carrot β-fructofuranosidase genes identification of a gene coding for a flower bud-specific isozyme[J].Plant Molecular Biology,1995,28：189-194.

[17]Godt D E,Roitsch T.Regulation and tissue specific distribution of mRNAs for three extracellular invertase isoenzymes of tomato suggests：an important function in establishing and maintaining sink metabolism[J].Plant Physiology,1997,115：273-282.


Memo

Memo:
-
Last Update: 2014-08-08