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《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2014年01期

Page:

22-25

Research Field:

Publishing date:

Info

Title:

Simultaneous Determination of 4 Endogenous Hormones in Chinese Chestnut Floral Bud by Solid Phase Extraction-High Performance Liquid Chromatography

Author(s):

LI Lin-ling; CHENG Hua; WU Cong-hua; HUA Juan; CHENG Shui-yuan

Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratories,Huanggang Normal University,Huanggang,Hubei 438000

Keywords:

solid phase extraction; high performance liquid chromatography; Chinese chestnut; floral bud; endogenous hormones

PACS:

S 664.2

DOI:

-

Abstract:

With Chinese chestnut floral bud as materials,an analytical method based on solid phase extraction-high performance liquid chromatography (SPE-HPLC) was established for the determination of endogenous hormones IAA,ABA,GA₃ and ZR in chestnut bud.The results showed that,80% precooled methanol aqueous solution was used to extract the buds endogenous hormones IAA,ABA,GA₃ and ZR from chestnut,and the result of extraction was better.Separation was performed on a Symmetry 300TM C₁₈ column (5 μm,4.6 mm×250 mm),using a mixture of methano and 0.075% glacial acetic acid solution (90∶5,volume ratio) as mobile phase at a flow rate of 1.0 mL/min.The ultraviolet detection was set at 219 nm,column temperature at 30°C,gradient elution,the separating effect was good,and the recovery ranged from 89.92% to 105.30%.The method was proved to be accurate,sensitive and reproducible.

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Last Update: 2014-08-07