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Effects of Two Types of Gelling Agents on the Callus Proliferation and Plant Regeneration of Citrus sinensis L.Osbeck cv.Bingtang(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年22期
Page:
108-110
Research Field:
Publishing date:

Info

Title:
Effects of Two Types of Gelling Agents on the Callus Proliferation and Plant Regeneration of Citrus sinensis L.Osbeck cv.Bingtang
Author(s):
LI Song-li; HUANG Huan-huan; CAI Xiao-dong
College of Horticulture and Gardening,Yangtze University,Jingzhou,Hubei 434025
Keywords:
Citrus; gelling agent; callus; plant regeneration; Gelrite
PACS:
S 666.2
DOI:
-
Abstract:
Taking embryogenic callus of Citrus sinensis L.Osbeck cv.Bingtang as initial explants,the effects of two types of gelling agents,agar and gelrite on the callus proliferation,somatic embryoid induction,bud induction and rooting in vitro was studied.The results showed that the culture medium prepared with agar was helpful for improving the proliferation rate of the embryogenic callus,and the culture medium prepared with gelrite facilitated somatic embryoid induction,bud induction and rooting of the embryogenic callus.Therefore,agar may be used during the process of callus culture,and it may be more reasonable of using gelrite as a gelling agent at the stages of somatic embryogenesis,bud induction and rooting.

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Last Update: 2014-08-07