|Table of Contents|

Study on Tissue Culture and Rapid Propagation of Salvia(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年21期
Page:
123-126
Research Field:
Publishing date:

Info

Title:
Study on Tissue Culture and Rapid Propagation of Salvia
Author(s):
FANG Shi-mei; LEI Shi-jun; WANG Hong-bo; WANG Yu; DING Xue-zhen
Weifang Vocational College，Weifang，Shandong 261041
Keywords:
Salvia; tissue culture; rapid propagation
PACS:
S 567.5+3
DOI:
-
Abstract:
Taking the explants of the stem tips，stem fragment，leaf，bud of Salvia as materials，the effect of different sterilizer time，different hormone levels on callus formation，buds differentiation，rooting，and plantlets acclimatization of Salvia were studied by random permutation test design method.The results showed that stems sterilized with 0.1% HgCl2 solution for 10 min could reach the highest survival rate of more than 90%；Salvia seedlings buds in MS+6-BA 1.0 mg/L+NAA 0.1 mg/L could get the best effect；on 1/2MS+NAA 0.4 mg/L the plantlets had the best rooting rate of more than 95%；plantlets acclimatization to a combination of vermiculite and perlite both water and breathable was more favorable for Salvia rooting，the survival rate could up to 98%.

References:

[1]李建秀,孙秀霞,周凤芹,等.山东丹参类药用植物新资源[J].山东中医学院学报,1995,19(3):190-191.

[2]温春秀，谢晓亮，吴志明，等.丹参脱病毒及组培快繁技术研究[J].中草药,2009(9):1058-1059.

[3]王维婷,单成钢,房翠萍,等.丹参组织培养及再生体系的建立与优化[J].现代中药研究与实践,2011,25(4):21-23.

[4]谢晓红,李江辉,冯文龙,等.丹参组培快繁技术研究[J].中药材,2004,27(7):474-475.

[5]张跃非,雷家容,代其林.丹参的组织培养及快速繁殖[J].植物生理学通讯,2003,39(2):139.

[6]梁红,何宇清,赵洁.生长调节物质对丹参叶片脱分化及根芽分化的效应[J].华中师范大学学报(自然科学版),1997,31(3):328.

[7]赵洁,陈志胜,万钧.丹参叶片无性系快速繁殖及植株再生研究[J].华中师范大学学报(自然科学版),1999,33(1):108.

[8]蔡朝辉,高山林,徐德然.丹参组织培养快速繁殖技术的研究[J].中国药科大学学报,1991,22(2):65.

[9]朱蔚华,胡秋.丹参组织培养研究[J].中药材,1994,17(4):3.

[10]田宇红,王吉之.丹参组织培养及植株再生研究[J].陕西师范大学学报(自然科学版),2003,31(1):99.

[11]陶璐璐,袁静明.丹参愈伤组织细胞固定化及其转化产物的特征[J].生物工程学报,1990,6(3):218.


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Last Update: 2014-08-27