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Gene Cloning and Sequence Analysis of Acid Invertase Gene in Magnolia(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年21期
Page:
120-122
Research Field:
Publishing date:

Info

Title:
Gene Cloning and Sequence Analysis of Acid Invertase Gene in Magnolia
Author(s):
CAO Chun-yan; ZHU Guang-hui; HUANG Rui
College of Horticulture and Technology,Soochow Polytechnic Institute of Agriculture，Soochow，Jiangsu 215008
Keywords:
Magnolia; invertase; cloning; sequence analysis
PACS:
S 685.15
DOI:
-
Abstract:
Taking RNA extracted from the petals of Magnolia as template，a pair of PCR primers were designed according to conservative sequence of acid invertase genes in other plants reported in GenBank，and acid invertase gene in Magnolia was cloned and its sequence was analyzed.The results showed that a 500 bp fragment was amplified.Sequence analysis showed that the fragment was 515 bp and encoded 171 amino acids.Homology analysis showed that nucleotide and amino acids sequence all shared high similarities with invertase genes cloned from other plants.Phylogenetic analysis revealed that this gene belonged to a class of acid invertase and located in the vacuole.

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Last Update: 2014-08-27