Study on Physiological Changs of Brassica juncea Coss.Under Drought Stress Simulated by PEG6000(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Physiological Changs of Brassica juncea Coss.Under Drought Stress Simulated by PEG6000

Author(s):: LI Wei-fen; QIN Rong; LIU Fa-wan; LI Fan; Institute of Horticulture,Yunnan Academy of Agricultural Sciences,Kunming，Yunnan 650205

Keywords:: Brassica juncea Coss.; seeding stage; drought resistance; PEG6000; simulated drought stress

Abstract:: Taking Yunnan local mustard variety ‘M100’ (Brassica juncea Coss.) as test material，the physiological changes under PEG6000 stress with the concentrations of 5%，8% and 10% were investigated.Physiological indexes such as the content of chlorophyll，MDA，proline，soluble sugar，relative water content，relative electric conductivity，Fv/Fm were determined with the prolongation of the time of PEG6000-induced stress.The results showed that with the increasing of PEG6000 concentration and the prolongation of treatment time，chlorophyll，relative water content，Fv/Fm of the leaf decreased obviously，and the content of MDA，proline，soluble sucrose，relative electric conductivity increased in varying degrees.Among these indexes，MDA content，proline content，soluble sugar content，relative water content，relative electric conductivity reached a significant difference between treatments under the concentration of 10% PEG6000 simulated drought stress，so these five indexes could be used for determination of mustard drought resistance.

[1]黄兴奇.云南作物种质资源(果树、油料、小宗作物、蔬菜篇)[M].昆明:云南科技出版社,2008.

[2]康绍忠.新农业科技革命与21世纪我国节水农业的发展[J].干旱地区农业研究,1998,16(1):11-17.

[3]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:260-261．

[4]祁云枝,杜勇军.干旱胁迫下黄瓜及蚕豆叶片膜透性改变及其机理的初步研究[J].陕西农业科学,1997(4):6-7.

[5]蔡小东,郭见林.PEG模拟干旱胁迫下辣椒相关生理指标的变化[J].湖南农业科学,2008(6):49-50,58.

[6]李志刚,刘威,林彰文,等.芦笋在PEG模拟干旱条件下的生理生化变化[J].生态科学,2006,25(1):21-23.

[7]宋唯一.PEG6000模拟干旱胁迫对黄瓜幼苗生长的影响[J].湖北农业科学,2011,50(12):2394-2397.

[8]王荣华,石雷,汤庚国,等.渗透胁迫对蒙古冰草幼苗保护酶系统的影响[J].植物学通报,2003,20(3):330-335.

[9]王霞,候平.植物对于旱胁迫的适应机理[J].干旱区研究,2001,18(2):42-46.

[10]李德全,郭清福,张以勤,等.冬小麦抗旱性生理特性的研究[J].作物学报,1993,19(2):125-131.

[11]任丽花,王义祥,翁伯琦,等.土壤水分胁迫对圆叶决明含水量和光合特性的影响[J].厦门大学学报(增刊),2005,44:28-31.

[12]芮仁廉，聂毓琦，童红玉.超氧物歧化酶（SOD）作为甘薯种质源抗逆性参数的探讨[J].江苏农业学报，1990，6（4）：52-56.

[13]肖用森.在渗透胁迫下稻苗中游离脯氨酸的积累与膜质过氧化的关系[J].武汉植物学研究,1996,14(4):334-340.

[14]姚觉,于晓英,邱收,等.植物抗旱机理研究进展[J].华北农学报,2007,22(增刊):51-56.

[15]韦小丽,徐锡增,朱守谦.水分胁迫下榆科3种幼苗生理生化指标的变化[J].南京林业大学学报(自然科学版),2005,29(2):47-50.

Last Update: 2014-08-26