|Table of Contents|

Study on the Biological Characteristics of Nine Cordyceps militaris Strains(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年20期
Page:
140-143
Research Field:
Publishing date:

Info

Title:
Study on the Biological Characteristics of Nine Cordyceps militaris Strains
Author(s):
LIU YangSUN Lai-yuLU Yun-huaHAN Zhi-ping
hool of Life Sciences,Huzhou Normal University,Huzhou,Zhejiang 313000
Keywords:
Cordyceps militarisbiological characteristicsITScordycepin
PACS:
S 567
DOI:
-
Abstract:
The biological characteristics of nine Cordyceps militaris strains were studied.The ITS sequence of the nine strains were analyzed and the cordycepin concentration in the mycelium and fermentation broth were also determined.The results showed that strain ‘GIM 5.502’,‘GIM 5.512’,‘GIM 5.270’,‘GIM 5.272’,‘GIM 5.266 ’could change color in mycelium normally,but they could not develop fruit body regularly,the other four strains could neither change color in mycelium normally,nor form fruit body.The ITS sequence analysis showed that it had little change between different Cordyceps militaris strains.The cordycepin concentration in the mycelium and fermentation broth of the strain‘GIM 5.502’ were the highest among that of the other strains,which was up to 6.971 mg/L,8.461 mg/L respectively.However,the cordycepin could not be assayed in that of strain‘GIM 5.267’,‘GIM 5.272’.

References:

[1]Paterson R R.Cordycepsa traditional Chinese medicine and another fungal therapeutic biofactory?[J].Phytochemistry200869(7)1469-1495.

[2]Das S KMasuda MSakurai Aet al.Medicinal uses of the mushroom Cordyceps militariscurrent state and prospects [J].Fitoterapia201081(8)961-968.

[3]彭国平,李红阳,袁永泰.冬虫夏草与人工蛹虫草的成分比较[J].南京中医药大学学报,1996(5)27-28,65.

[4]秦秀丽,杨国会,李风林.蛹虫草液体深层发酵的研究[J].北方园艺,2010(23)167-170.

[5]徐芳旭,刘诗扬,王升厚.蛹虫草液体菌种培养基的优化[J].北方园艺,2011(4)199-201.

[6]周德庆,微生物学实验教程[M].2.北京:高等教育出版社,200656-63.

[7]梁月,张国珍,安沫平,等.蛹虫草子囊孢子萌发及其后代群体培养性状观察[J].菌物学报,200524(4)525-532.

[8]朱衡,瞿峰,朱立煌.利用氯化苄提取适于分子生物学分析的真菌 DNA[J].真菌学报,199413(1)34-40.

[9]高新华.蛹虫草 (Cordyceps militaris) 的交配型研究[J].食用菌学报,200815(1):1-5.

[10]李美娜,吴谢军,李春燕,等.人工栽培蛹虫草(Cordyceps militaris)性状变异的遗传学分析[J].菌物系统,200322(2):277-282.


Memo

Memo:
-
Last Update: 2014-08-27