Taking Dendrobium chrysotoum as material,the effect of hormones kinds and concentrations,plant medias and nutrient solutions on germination and growth of new branch of Dendrobium chrysotoum were studied.The results showed that taking hormone immerse the base of D.chrysotoum could promote new branch germination and growth in a certain extent,but different hormones had different effects.Plant media had great influence on new branch germination and growth,familiar bark plus water moss was the best choice,the next was water moss,snakewood plus ceramsite.Nutrient solution had the same effect on growth of new branch,compound fertilizer was the best.