|Table of Contents|

Comparison of Different Methods for Extracting Genomic DNA of Synechococcus sp.PCC 7002(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年20期
Page:
95-97
Research Field:
Publishing date:

Info

Title:
Comparison of Different Methods for Extracting Genomic DNA of Synechococcus sp.PCC 7002
Author(s):
DONG Xue-wei1; LI You-zhi1; HE Qing-fang2; 3; YU Xiu-min4; PENG Zhen-ying2; BI Yu-ping2
1.College of Life Science and Technology，State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources，Guangxi University，Nanning，Guangxi 530004；
2.High-Tech Research Center，Shandong Academy of Agricultural Science，Key Laboratory for Genetic Improvement of Crop，Animal and Poultry of Shandong Province，Key Laboratory of Crop Genetic Improvement and Biotechnology，Huanghuaihai，Ministry of Agriculture，Jinan，Shandong 250100；


Keywords:
Synechococcus sp.PCC 7002; genomic DNA; extraction method
PACS:
Q 949.22
DOI:
-
Abstract:
Taking Synechococcus sp.PCC 7002 as material，four methods of high-salt method，CTAB method，PVP method and quartz sand method were used to extract the genomic DNA of it，and the concentration and purity of DNA were detected by nucleic acid analyzer，agarose condensate gel electrophoresis and PCR amplification were analyzed.The results showed that the four methods were able to extract genomic DNA from Synechococcus sp.PCC 7002，the DNA which was extracted by PVP method had the highest purity，containing less protein and RNA，the high-salt method and the quartz sand method followed，the CTAB method had the lowest purity.The DNA which was extracted by the quartz sand method had the highest concentration，the PVP method followed，then the high-salt method，the CTAB method had the minimum concentration.The genomic DNA which was extracted by four kinds of method could amplify the ideal band.

References:

[1]马英,焦念志.聚球藻(Synechococcus)分子生态学研究进展[J].自然科学进展,2004,14(9):967-971.

[2]Batterton J C Jr，Van Baalen C.Growth responses of blue-green algae to sodium chloride concentration[J].Archiv für Mikrobiologie,1971,76(2):151-165.

[3]Nomura C T，Sakamoto T，Bryant D A.Roles for heme-copper oxidases in extreme high-light and oxidative stress response in the cyanobacterium?Synechococcussp.PCC 7002[J].Archiv für Mikrobiologie,2006,185(6):471-479.

[4]Stanier R Y，Cohen B G.Phototrophic prokaryotes：the cyanobacteria[J].Annual Reviews in Microbiology,1977,31(1):225-274.

[5]陈斌.蓝藻对黄芩苷生物转化研究初探[D].北京:北京中医药大学,2009.

[6]Stevens S E，Porter R D.Transformation in Agmenellum quadruplicatum[J].Proceedings of the National Academy of Sciences of the United States of America,1980,77(10):6052-6056.

[7]Frigaard N U，Sakuragi Y，Bryant D A.Gene inactivation in the cyanobacteriumSynechococcussp.PCC 7002 and the green sulfur bacterium Chlorobium tepidum using in vitro-made DNA constructs and natural transformation[J].Methods In Molecular Biology,2004,274(3):325-340.

[8]Xu Y，Alvey R M，Byrne P O，et al.Expression of genes in cyanobacteria：adaptation of endogenous plasmids as platforms for high-level gene expression in?Synechococcussp.PCC 7002[J].Methods In Molecular Biology,2011,684(2):273-293.

[9]Salah M A，Iciar M.Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques[J].Nucleic Acids Reserch,1997,22(25):4692-4693.

[10]杨泽民,章群,谢数涛,等.金藻基因组DNA的提取与PCR扩增[J].生物学杂志,2008,25(1):60-63.

[11]陈高.多不饱和脂肪酸生物合成途径相关酶基因的克隆及在集胞藻PCC6803中的表达研究[D].济南:山东师范大学,2012.

[12]马艳芝,张玉星.梨属植物基因组DNA提取部位对RAPD扩增的影响[J].西南农业学报,2009,22(4):1042-1045.

[13]刘昔辉,宋焕忠,张革民,等.一种快速高效提取甘蔗及其近缘属基因组DNA的方法[J].西南农业学报,2010,23(2):515-518.

[14]杜金子,陈丽梅,黄荣韶,等.改良SDS法提取石韦基因组DNA的研究[J].西南农业学报,2009,22(3):754-758.

[15]Fu R Z，Wang J，Sun Y R，et al.Extraction of genomic DNA suitable for PCR analysis from dried plant rhizomes/roots[J].BioTechniques,1998,25(11):7962-7968.

[16]徐伟丽,马莺,杜明,等.豆粉基因组DNA不同提取方法的比较[J].中国农学通报,2011,27(23):119-122.


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Last Update: 2014-08-26