|Table of Contents|

Cloning and Expression Analysis of WRKY Gene Segment in Lycium barbarum L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年20期
Page:
89-94
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of WRKY Gene Segment in Lycium barbarum L.
Author(s):
WANG Li-juanGAO Wei-fengGUAN Cui-pingWANG Yan-cai
Ningxia University,Yinchuan,Ningxia 750021
Keywords:
Lycium barbarum L.WRKY transcription factorgene cloningexpression analysis
PACS:
Q 785
DOI:
-
Abstract:
Taking Lycium barbarum L.as material,primers were designed according to WRKY conserved transcription factor fragment form known species.Lycium barbarum L.WRKY gene fragments were cloned by RT-PCR method.Sequence of the fragments obtained was analyzed.The expression of Lycium barbarum L.in various organs was identified by Real-time PCR.They all contributed to research the function of WRKY transcription factor of Lycium barbarum L.The results showed that the cDNA fragment was 467 bp and named LbWRKY (GenBank:KF181204).Sequential analysis indicated that the sequence contained WRKYGQK conservative domain,and the similarity with Solanum lycopersicum WRKY and Nicotiana tabacum wizz were 95% and 91%,respectively.The results showed that WRKY gene sequence in Lycium barbarum L.was cloned successfully.Real time PCR analysis showed that the LbWRKY gene was highly expressed in fruit.The expression level reached the lowest level in leaf.LbWRKY didn’t express in root.

References:

[1]Ishiguro SNakamura K.Characterization of a cDNA en-coding a novel DNA-binding proteinSPF1that recognizes SP8 sequences in the 5, upstream?regions of genes coding for sporamin and beta-amylase from sweet potato[J].Mol Gen Genet,1994,244(6):563-571.

[2]Rushton P JMacdonald HHuttly A Ket al.Members of a new family of DNA-binding proteins bind to a conserved cis-element in the promoters of a-Amy2 genes[J].Plant Mol Biol,1995,29(4):691-702.

[3]RushtonP JTorres J TParniske Met al.Interaction of elicitor-inducedDNA-binding proteins with elicitor response elements in the promoters?of parsley PR1 genes[J].EMBO J,1996,15(20):5690-5700.

[4]dePater SGreco VPham Ket al.Characterization of a zinc-dependent transcriptional activator from Arabidopsis[J].Nucleic Acid Res,1996,24(3):4624-4631.

[5]Yoda HOgawa MYamaguchi Yet al.Identification of early-responsive genes associated with the hypersensitive response to tobacco mosaic virus and characterization of a WRKY-type transcription factor in tobacco plants[J].Mol Genet Genomics,2002,267(2):154-161.

[6]Dong J XChen C HChen Z X.Expression profiles of the ArabidopsisWRKY gene super family during plant defense response[J].Plant Mol Biol,2003,51(1):21-37.

[7]Qiu Y PJing S JFu Jet al.Cloning and analysis of expression profile of 13 WRKY genes in rice[J].Chin Sci Bull,2004,49(20):1860-1869.

[8]Eulgem TRushton P JRobatzek Set al.The WRKY super family of plant transcription factors[J].Trends Plant Science,2000(5):199-206.

[9]Turck FZhou ASomssich I E.Stimulus-dependentpromoter specific binding of transcription factor WRKY1 to its native promoter and the defense-related gene PcPR1-1 in parsley[J].Plant Cell,2004,16(10):2573-2585.

[10]Zhang Y JWang L J.The WRKY transcription factor super family its origin in eukaryotes and expansion in plants[J].BMC Evol Biol,2005,5(1):1-12.

[11]Kalde MBarth MSomssich I Eet al.Members of the ArabidopsisWRKY Group transcriptional factors are part of different plant defense signaling pathways[J].Mol Plant Microbe Interact,2003,16(4):295-305.

[12]白寿宁.宁夏枸杞研究[M].银川:宁夏人民出版社,1999:29-30.

[13]郑国琦,罗霄,买立芸,.不同品种宁夏枸杞果实糖积累差异的生理原因[J].农业科学研究,2009,30(1):1-4.

[14]Johnson C SKolevski BSmyth D R.Transparent test a GLABRA2a trichome and seed coat development gene of Arabidopsisencodes a WRKY transcription factor[J].Plant Cell,2002,14(6):1359-1375.

[15]Zhang Z LXie ZZou Xet al.A rice WRKY gene encodes a transcriptional repressor of the gibberell in signaling path way in aleurone cells[J].Plant Physiol,2004,134(4):150-1513.

[16]Wu K LGuo Z JWang H Het al.The WRKY family of transcription factors in rice and Arabidopsisand their origins[J].DNA Res,2005,12(1):9-26.

[17]贾翠玲,侯和胜.植物WRKY转录因子的结构特点及其在植物防卫反应中的作用[J].天津农业科学,2010(2):21-26.

[18]Aoki KYano KSuzuki Aet al.Large-scale analysis of full length cDNAsfrom the tomato (Solanum lycopersicum) cultivar Micro-Tom a reference?system for the?Solanaceaegenomics[J].BMC Genomics,2010,11(3):1471-2164.

[19]Sun?CPalmqvist SOlsson Het al.A novel WRKY transcription factorSUSIBA2participates in sugar signaling in barley by binding to the sugar-reponsive elements of the isol promoter[J].Plant Cell,2003,15(9):2076-2092.

[20]Shree P PImre E S.The role of WRKY transcription factors in plant immunity[J].Plant Physiol,2009,150(4):1648-1655.

[21]Xu Y HWang J WWang Set al.Characterization of GaWRKY1a cotton transcription factor that regulates the sesquiterpene synthasegene(+)-dalta-cadinene synthase-A[J].Plant Physiol,2004,135(1):507-515.


Memo

Memo:
-
Last Update: 2014-08-26