Optimization of Liquid Culture Medium of Armillaria mellea Exopolysaccharides(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Optimization of Liquid Culture Medium of Armillaria mellea Exopolysaccharides

Author(s):: WEN Gang; LIU Hai-yan; YANG Mei; School of Environmental and Biological Engineering，Jilin Institute of Chemical Techonlogy，Jilin，Jilin 132022

Keywords:: Armillaria mellea; exopolysaccharides; culture medium; orthogonal test

Abstract:: Taking Armillaria mellea as material,the effect of carbon source，nitrogen source and inorganic ion on the yield of exopolysaccharides were studied，and the liquid culture medium of it was optimized by orthogonal test.The results showed that the optimal culture medium was：sucrose 25.00 g/L，peptone 2.50 g/L，MgSO₄ 0.50 g/L，K₂HPO₄ 1.50 g/L，KH₂PO₄ 0.25 g/L，ZnSO₄ 0.03 g/L，and FeSO₄ 0.02 g/L.In these conditions，the exopolysaccharides of Armillaria mellea can reach 478.5±22.4 mg/L，it was 6 times as the mycelium biomass of the basal medium.

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Last Update: 2014-08-26