Studying on Antifungal Compounds from Root Extracts of Stellera chamaejasme Against Plant Pathogenic Fungi(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2013年19期

Page:

120-123

Research Field:

Publishing date:

Info

Title:

Studying on Antifungal Compounds from Root Extracts of Stellera chamaejasme Against Plant Pathogenic Fungi

Author(s):

BU Chun-ya¹; 2; CHENG Jun²; WANG You-nian¹; 2; GONG Yi-wen¹; WANG Chong-qing¹; SHI Guang-lu¹; 2

1.College of Biological Science and Engineering，Beijing University of Agriculture，Beijing 102206；

2．Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture P.R.China，Beijing University of Agriculture，Beijing 102206

Keywords:

Stellera chamaejasme; Botrytis cinerea Pers.; antifungal compounds; chamaechromone

PACS:

S 482.2+92

DOI:

-

Abstract:

Taking Botrytis cinerea Pers.,Colletotrichum acutatum，Monilinia fructicola，Fusarium moniliforme and Pythium coeruleum as test fungi，screening antifungal compounds from the root crude extracts of Stellera chamaejasme,and separated the major antifungal compounds from Stellera chamaejasme through adapting the macroporous resin，which was suitable for the large-scale separation,the antifungal compounds extraction process were established.The results showed that the ethanol extraction followed by ethyl acetate partition，could better isolate antifungal compounds from root of S.chamaejasme.The antifungal components of ethyl acetate extract of S.chamaejasme were further efficiently partitioned using the macroporous resin.The 9th elution fraction eluted from macroporous resin column was found to have highest antifungal activity against B.cinerea with an IC₅₀ value of 0.39 mg/mL.The main component of the 9th fraction of S.chamaejasme was separated and identified as chamaechromone using HPLC and NMR.Chamaechromone was first found to be able to effectively inhibit the growth of mycelium of B.cinerea，C.acutatum，and M.fructicola.

References:

[1]谢小梅，许杨．抗真菌中药的作用机理研究进展[J]．中国中药杂志，2004，29(3)：200-202.

[2]温哲屹，师光禄，苏学友，等．瑞香狼毒提取物对病原菌及桃酶的生物活性研究[J]．北京农学院学报，2008，23(1)：25-29.

[3]Feng B M，Wang T，Zhang Y，et al.Aldose reductase inhibitors from Stellera chamaejasme[J].Pharmaceutical Biology，2005，43(1)：12-14.

[4]Yang G H，Chen D F．Billavanones，flavonoids，and coumarins from the roots of Stellera chamaejasme and their antiviral effect on hepatitis B virus[J]．Chemistry and Biodiversity，2008，5(7)：1612-1872.

[5]张国洲，王亚维，徐汉虹，等．瑞香狼毒杀虫活性成分的提取与分离(Ⅰ)[J]．安徽农业大学学报，2000，27(4)：340-344．

[6]张国洲，王亚维，徐汉虹，等．瑞香狼毒杀虫活性成分的提取与分离(Ⅱ)[J]．安徽农业大学学报，2000，27(4)：345-347.

[7]龚晓霞，李文娟，欧阳秋，等．瑞香狼毒根中抑菌活性成分的研究[J]．四川大学学报(自然科学版)，2006，43(3):697-701.

[8]Niwa M，Liu G Q，Tatematsu S,et al．Chamaechromone，a novel rearranged biflanvonoid from Stellera chamaejasme L.[J].Tetrahydron Letters，1984，25(34)：3735-3738.

[9]Niwa M，Otsuji S，Tatematsu S，et al.Stereostructures of two biflavanones from Stellera chamaejasme[J].L Chem Pharm Bull，1986，34：3249-3251.

[10]李淑珍，李进，杨志江，等．大孔树脂分离纯化黑果枸杞总黄酮的研究[J].食品科学，2009，30(1)：19-24.

[11]秦宝福，周乐，苗芳，等．瑞香狼毒根的抑菌活性研究（Ⅰ）[J].西北植物学报，2003，23(11)：1977-1980.

[12]张国洲，徐汉虹，王亚维，等．瑞香亭与狼毒色原酮对昆虫的拒食和胃毒作用[J]．青海大学学报，2001，19(6)：1-3.

[13]王玉华，杨夏，孙丽君，等.蒙药材瑞香狼毒体外细胞毒性的研究[J].中国医药生物技术，2012，7(1)：9-13.

[14]刘耀红，李福华，周乐，等.瑞香狼毒根丙酮提取物对几种动物病原菌的抑制作用[J].西北农业学报，2008，17(6):184-186.

[15]张武岗，李定刚，宋毓民，等.新狼毒素B及其衍生物的抑菌活性研究[J].动物医学进展，2005，26(11):66-69.

Memo

Memo:

-

Common functions