Study on Mutugenesis of Tetraploid Watermelon by Using Oryzalin(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: ZHANG Na; SHI Xian-feng; REN Jian; ZENG Hong-xia; CHENG Wei-shun; SUN Yu-hong; Wuhan Institute of Agricultural Sciences，Wuhan，Hubei 430345

Keywords:: small-type watermelon; yellow-flesh; tetraploid; mutugenesis; Oryzalin

Abstract:: In order to provide breeding high-quality parent material for seedless watermelon，taking small-type diploid watermelon line A8 as material，tetraploid plants of watermelon were induced by treating the growing points of seedlings with different concentration Oryzalin and lasting different treating time.Autotetraploid plants were selected and identified basing on morphology，flow cytometry，hybridization and characterization.Then observed a stable tetraploid material after three quarters’ continuous cultivation，observation and filtering.The results showed that different treatments could obtain tetraploid watermelons by processing with Oryzalin.The most effective way was extirpating interiorleaf at the 8th day after sowing，then treating the growing points of seedlings with 100 mg/L Oryzalin for 6 d.The treatmented seedlings could all survival and the induction frequency of tetraploid watermelon was 30.59%.

[1]刘文革.我国无籽西瓜产业发展状况与对策[J].长江蔬菜,2010(8):121-127.

[2]王惠林,张慧林,周志成.ORYZALIN不同处理方法对西瓜四倍体诱变效果的影响[J].新疆农业大学学报,2008,31(6):21-25.

[3]中国农业科学院果树研究所郑州分所.培育四倍体西瓜初报[J].植物学通讯,1974(2):26-31.

[4]袁建民.小果型西瓜离体培养关键技术及四倍体诱导研究[D].海口:海南大学,2010:5-7.

[5]王秀莉,赵世萍.秋水仙素诱导细胞周期停滞[J].生物学通报,2010,45(8):51-52.

[6]谭素英,刘济伟,黄秀强,等.西瓜新品种‘蜜枚四倍体’的选育研究[J].果树科学,1993,10(2):87-91.

[7]祁碧菽,罗耀武,任清.四倍体葡萄花粉母细胞减数分裂与坐果率关系的研究[J].河北农业大学学报,2000,23(4):49-52.

[8]施先锋,彭金光,汪李平.用西瓜叶片气孔保卫细胞叶绿体数鉴定西瓜染色体倍性[J].湖南农业大学学报(自然科学版),2009,35(6):640-643.

Last Update: 2014-08-26