|Table of Contents|

Comparison on Genomic DNA Extracted from the Leaves of Chinese Chestnut by Two Methods(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年18期
Page:
89-91
Research Field:
Publishing date:

Info

Title:
Comparison on Genomic DNA Extracted from the Leaves of Chinese Chestnut by Two Methods
Author(s):
CHEN Juan1; 2; JIN Wen-long1; MO Yi-wei1; 3
1.Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization,Huanggang Normal University,Huanggang,Hubei 438000;
2.College of Chemistry and Life Science,Huanggang Normal University,Huanggang,Hubei 438000;
3.College of Life Science,Shaoxing University,Shaoxing,Zhejiang 312000
Keywords:
Chinese chestnut; DNA extraction; modified CTAB method; extraction Kits; DNA quality
PACS:
S 603.6
DOI:
-
Abstract:
New leaves of nine Castanea mollissima Blume varieties from Luotian country Hubei province were used as experimental materials and genomic DNA extraction methods of plant genomic DNA Kit and modified CTAB were used and compared.The results showed that the method of plant genomic DNA Kit was poor and was difficult to obtain clear bands by agarose gel electrophoresis.The ratio A260/A280of extracted DNA was 1.11~1.49 and the yield was 24.50~59.25μg/g.However,PVP and 2-mercaptoethanol were added to CTAB buffer in order to inhibit oxidation of polyphenol,and elution for many times were used to remove polysaccharide could be inhibited and most of polysaccharides in leaves could be removed efficiently.The ratio A260/A280of DNA extracted by modified CTAB ranged from 1.80~1.90,and the yield was 60.50~102.00 μg/g.The result of Castanea mollissima Blume molecular marker also showed that the modified CTAB method was suitable for extracting high qualified DNA from Castanea mollissima Blume.

References:

[1]   暴朝霞,黄宏文.板栗主栽品种的遗传多样性及其亲缘关系分析[J].园艺学报,2002,29(1):13-19.
[2]   程丽莉,苏淑钗,秦岭,等.板栗叶片DNA的提取及AFLP反应体系的建立[J].北京农学院学报,2005,20(2):5-9.
[3]   王同坤,董超华,马艳,等.板栗SSR和RAPD技术体系的建立[J].果树学报,2006,23(6):825-829.
[4]   艾呈祥,余贤美,张力思,等.中国部分板栗品种的SSR 标记[J].农业生物技术学报,2007,15(2):283-289.
[5]   徐育海,蒋迎春,方波,等.罗田县板栗产业生产调查[J].湖北农业科学,2008,47(1):67-70.
[6]   王晴芳,徐育海.湖北大别山区板栗产业可持续发展探讨[J].湖北农业科学,2011,50(23):4485-4487.
[7]   姜德志,程水源,王燕,等.罗田3个板栗栽培品种主要营养成分分析[J].湖北农业科学,2011,50(23):4482-4485.
[8]   邱文武,孙伟生,窦美安.菠萝SRAP反应体系的建立及优化[J].生物技术,2008,18(1):39-42.
[9]   Li G,Quiros C F.Sequence-related amplified polymorphism (SRAP),A new marker system based on a simple PCR reaction its application to mapping and gene tagging in Brassica[J].Theoretical and Applied Genetics,2001,103:455-461.
[10] 陈惠云,孙志栋,茅轶俊,等.春兰基因组DNA提取方法的研究[J].分子植物育种,2006,4(1):135-142.
[11] 刘婵,王博,段青,等.滇山茶基因组DNA不同提取方法效果比较[J].江苏农业科学,2010(6):49-50.
[12] 胡凤荣,任翠,鲍仁蕾,等.风信子DNA不同提取方法的效果比较[J].沈阳农业大学学报,2011,42(5):570-573.

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Last Update: 2014-08-14