|Table of Contents|

Detection and Analysis of Microsatellites in the Phytophthora infestans Genome(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年17期
Page:
110-114
Research Field:
Publishing date:

Info

Title:
Detection and Analysis of Microsatellites in the Phytophthora infestans Genome
Author(s):
ZHANG Hong-lei; ZHU Jie-hua; HU Zhen-zhu; YANG Zhi-hui
Department of Plant Pathology,Agricultural University of Hebei,Baoding,Hebei 071000
Keywords:
Phytophthora infestans; genome; microsatellite
PACS:
Q 813
DOI:
-
Abstract:
The amount and distribution of pure and compound microsatellite in the whole genome and its different regions of Phytophthora infestans were identified by software SciRoKo.The results showed that,a total of 5 010 loci for perfect microsatellites were detected in the genome,whose density was 26.35 SSRs/Mb,and the perfect motifs were ACGG,AT and AAG.Only motif for 3 to 6 bases occurred in the exons,in which the percent of microsatellites with 3 and 6 bases motif accounted for 93.68%.The number of compound microsatellites were 275,accounting for 82.18% was interrupted perfect microsatellites.The loci suitable for microsatellite markers were identified according to the software of SciRoKo.At last,430 loci suitable for the development of microsatellites were acquired,in which the coding regions were 83 and non-coding regions were 264.In the coding region and non-coding regions,which were respectively selected 32 and 105 microsatellite loci for validation.The results showed that the success rate of its development and polymorphism significantly increased.This provided a reliable method for the development of microsatellite in genome of high repetition.

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Last Update: 2014-08-11