|Table of Contents|

Optimization and Establishment of SRAP-PCR Reaction System of Vaccinium ssp. and Analysis of the Genetic Diversity(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年17期
Page:
95-99
Research Field:
Publishing date:

Info

Title:
Optimization and Establishment of SRAP-PCR Reaction System of Vaccinium ssp. and Analysis of the Genetic Diversity
Author(s):
FU Bing1; HONG Zhen1; LIU Yue-jun2; PAN Zhi-mei1
1.Branch of Environment Engineering,Lishui Vocational and Technical College,Lishui,Zhejiang 323000;
2.Lishui Forestry Institute of Zhejiang,Lishui,Zhejiang 323000
Keywords:
blueberry; SRAP-PCR; optimization; genetic diversity
PACS:
S 663.9
DOI:
-
Abstract:
Taking 9 varieties of blueberry as material,the SRAP-PCR reaction system of Vaccinium ssp. was established and optimized by L16(45) orthogonal experimental design.The genetic diversity of them was analyzed by the optimal reaction system.The results showed that the optimal and stable SRAP-PCR system was as follows:2.5 mmol/L Mg2+,100 μmol/L dNTPs,0.8 U Taq polymerase,0.2 μmol/L primers and 40 ng DNA template,1×Reaction Buffer,within total 20 μL reaction solution.The GS coefficients among the 9 varieties of blueberry were ranged from 0.1818 to 1.0000 with an average of 0.6359,this suggested that the genetic variation was high.These varieties were divided into four groups on the UPGMA dendrogram constructed from GS coefficients,and also divided into four groups by the method of principal coordinates analysis.The UPGMA and GS coefficients results was the same.

References:

[1]  尹德洁.蓝莓野生资源和SRAP遗传多样性研究[D].北京:北京林业大学,2012.
[2]  黄春辉,夏思进,曲雪艳,等.蓝莓的栽培利用现状与发展前景[J].现代园艺,2011(6):41-43.
[3]  茨韦特科夫?约旦,赵燕丽,王春,等.引进国外蓝莓品种的组织培养及快繁技术研究[J].黑龙江农业科学,2011(6):6-8.
[4]  王程,罗军,田志杰,等.利用RAPD标记分析长白山越桔的遗传多样性及亲缘关系[J].吉林医药学院学报,2009,30(2):73-75.
[5]  崔建民,杨忠,邹荣仟,等.越橘SSR反应体系的优化[J].吉林农业大学学报,2009,31(5):506
[6]  杨云,孟慧,吴翼,等.降香黄檀SRAP分子标记的引物筛选[J].江西农业学报,2011,23(3):29-31.
[7]  杨晓东,郭立国,徐立功.SRAP在大白菜遗传多样性分析实验中的应用[J].中国园艺文摘,2010(10):16-18.
[8]  孙祖霞,刘兆磊,陈素梅,等.荷花SRAP-PCR 反应体系的优化与确立[J].南京农业大学学报,2011,34(6):53-58.

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Last Update: 2014-08-11