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Impacting Factors on Embryo Rescue of Seedless Grape with Cold Resistance(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年17期
Page:
11-15
Research Field:
Publishing date:

Info

Title:
Impacting Factors on Embryo Rescue of Seedless Grape with Cold Resistance
Author(s):
ZHAO Kai; LIU Qiao; ZHANG Jian-xia; NIU Ru-xuan; LI Gui-rong; WANG Yue-jin
Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China,State Key Laboratory of Crop Stress Biology in Arid Area,College of Horticulture,Northwest Agricultural and Forestry University,Yangling,Shaanxi 712100
Keywords:
seedless grape; cold resistance; germplasm; embryo rescue
PACS:
S 663.1
DOI:
-
Abstract:
Taking 7 cultivars of stenospermocarpic seedless grape as female,2 cultivars of interspecific hybrids as male,the embryo rescue was done with hybrid ovule inoculated on medium.Effect of different ovule culture media,supplementation of different amino acid and different genotype on embryo rescue invitro were studied using the ovules from 8 cross combinations.The results indicated that among three medias used for culturing ovules on ER+CH 500 mg/L+GA3 0.5 mg/L+IAA 1.5 mg/L medium got the highest germination rate and seedling ratio.But added CH and GA3+IAA had no appreciable impact on proportion of embryos developed and seeding ratio.Supplementation of different amino acid had different effect on the proportion of embryo development and seedling rate.Particularly,among all the tested amino ascids,Ser of 1 mmol/L had remarkable promoting effect in the cross combination of ‘Perlette’×hybrid ‘Beichun’ (V.vinifera cv.‘Muscat Hambug’×V.amurensis).While,supplement of Niacin in the media perform contrarily by inhibiting the development of the embryo.In six cross combinations which whose male parent was ‘00-1-5’(‘Muscat Hambug’×Vitis amurensis accession ‘Heilongjiang Seedling’),when stenospermocarpic seedless cultivar ‘Jingdajing’ (V.vinifera) as female parent it can obtain the largest proportion of embryo development and seedling rate.Among the two cross combinations of stenospermocarpic seedless cultivar ‘Royal Autumn’בBeichun’ and ‘Sultanina Rose’בBeichun’,‘Royal Autumn’ was good to be a female as well as ‘Sultanina Rose’.When used ‘Beichun’ as a male,it got higher embryo development rate and germination rate than ‘00-1-5’,but the result was not significant.Thirty-nine new seedless grape germplasms with cold resistant were obtained by embryo rescue technology.

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Last Update: 2014-08-08