|Table of Contents|

Establishment of High Frequency Regeneration System of Stevia rebaudiana Bertoni Leaf(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年09期
Page:
134-137
Research Field:
Publishing date:

Info

Title:
Establishment of High Frequency Regeneration System of Stevia rebaudiana Bertoni Leaf
Author(s):
CAI Yong-zhi; WANG Ai-ying; SHEN Hai-tao; ZHU Jian-bo
College of Life Science,Shihezi University，Shihezi，Xinjiang 832003
Keywords:
Stevia rebaudiana Bertoni; leaf; regeneration system
PACS:
S 681.903.6
DOI:
-
Abstract:
Taking young leaves of Stevia rebaudiana Bertoni as explants，the effects of disinfection time and exogenous hormones on the survival rate，induction of in vitro shoot，growth and root formation were studied.The results showed that the optimum disinfection method was treated with 0.1％HgCl2 for 3 min；the optimum adventitious buds induction medium from leaves was MS+2.0 mg/L 6-BA+1.0 mg/L IAA+300 mg/L CH (Casein Acid Hydrolysate) and the average induction rate was 80％；the optimum shoot growth medium was MS+0.5 mg/L 6-BA+0.05 mg/L NAA+0.2 mg/L GA3+4.0 mg/L KT；the optimum rooting medium was 1/2MS+0.2 mg/L IAA with rooting rate 100％.The tissue cultured plants obtained grew well and had high survival rate after transplanting，the optimum regeneration system of Stevia rebaudiana Bertoni was found finally.

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Last Update: 2014-08-25