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Preliminary Study on the Total DNA Extracted from Psidium guajava L. by Improved CTAB Method(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年09期
Page:
123-125
Research Field:
Publishing date:

Info

Title:
Preliminary Study on the Total DNA Extracted from Psidium guajava L. by Improved CTAB Method
Author(s):
ZHAO Zhi-chang; CHEN Ye-yuan; GAO Ai-ping; LUO Shi-rong; HUANG Jian-feng
Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China，Tropical Crops Genetic Resources Institute，Chinese Academy of Tropical Agricultural Sciences，Danzhou，Hainan 571737
Keywords:
improved CTAB method; Psidium guajava L.; DNA extraction
PACS:
S 667.9
DOI:
-
Abstract:
Taking Psidium guajava as material，total DNA was extracted from fresh Psidium guajava leaves by CTAB，SDS and improved CTAB method respectively，and it was detected by agarose gel electrophoresis and SCoT-PCR marker analysis.The results showed that the above three methods were available for DNA extraction from Psidium guajava leaf，but improved CTAB method by adding rinse and two times washing，could effectively remove polyphenols，polysaccharides，protein and other substances of samples.The high quality DNA was extracted from fresh leaves，and could be used for SCoT-PCR marker analysis.

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Last Update: 2014-08-25