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《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年09期
Page:
119-123
Research Field:
Publishing date:

Info

Title:
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Author(s):
Study on Identification of Ty-1 Gene; Ty-2 Gene; Mi Gene and Cf-5 Gene by Quadruple PCR in Tomato
LIU Chao-qin，LI Jing-fu，XU Xiang-yang，JIANG Jing-bin
Keywords:
tomato; multi-PCR;  Ty-1; Ty-2; Mi; Cf-5
PACS:
S 641.203.6
DOI:
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Abstract:
Taking Lycopersicon esculentum as material，Ty-1，Ty-2，Mi and Cf-5 in tomato tightly linked with four SCAR markers respectively resistant to Tomato Yellow Leaf Curl virus，root-knot nematode and tomato leaf mold were amplified and screened by using a single PCR reaction.The results showed that the PCR products were identical with one amplified by a single SCAR primer.Among them，four SCAR markers tightly linked with Ty-1，Ty-2，Mi and Cf-5 genes produced 395，900，750 and 960 bp bands.Cleavaged with the restriction enzyme Taq I，these specific fragments produced almost unanimously the same size with the others.Preliminary proved the veracity of quadruple PCR.The accurate results proved that Ty-1，Ty-2，Mi and Cf-5 resistant genes could be identified simultaneously by using under adaptable condition.Quadruple PCR could be very important for marker-assisted selection during early stage in tomato.Also could reduce production costs，time saving and less labor.If could reasonable used，it will become an important method of breeding work.

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Last Update: 2014-08-25