Study on Factors Influencing Plant Regeneration of Clivia miniata Leaf(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Factors Influencing Plant Regeneration of Clivia miniata Leaf

Author(s):: GUAN Li-xia; Department of Biotech，Liaoning Agricultural Vocation-technical College，Yingkou，Liaoning 115009

Abstract:: Taking the leaf of Clivia miniata as material，the effects of different culture mediums，different leaf parts and multiplication algebra on the plant regeneration in vitro was studied.The results showed that the in vitro Clivia miniata leaf base regeneration capacity was the strongest，and the best differentiation medium was MS+ZT 1.5 mg/L+KT 0.5 mg/L+NAA 0.5 mg/L；tissue culture proliferation 1～7 generation of the in vitro Clivia miniata leaf regeneration ability was stronger，then culture regeneration ability was abate，10 generations after the regenerative capacity of the weakest；the best rooting medium for the in vitro plant was 1/2MS+IBA 1.0 mg/L，with rooting rate more than 98%.

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Last Update: 2014-08-25