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Study on Cell Suspension Culture and Plant Regeneration of Torenia fournieri L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年09期
Page:
109-113
Research Field:
Publishing date:

Info

Title:
Study on Cell Suspension Culture and Plant Regeneration of Torenia fournieri L.
Author(s):
LIANG Zheng; LI Hou-hua; QUE Yi; FU Lin-jiang; LI Ling; GUO Yi-bo
College of Forestry，Northwest Agricultural and Forestry University，Yangling，Shaanxi 712100
Keywords:
Torenia fournieri L.; suspension culture; plant regeneration
PACS:
S 681.9
DOI:
-
Abstract:
Taking young leaves of Torenia fournieri ‘Clown’ series as material，the callus induction，suspension culture and plant regeneration were studied.The results showed that MS medium with supplement of 1.0 mg/L 6-benzylamino purine (6-BA) and 0.1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) efficiently induced rapid-growing and loose callus.The obtained callus was taken as suspension culture material.The most suitable conditions for the establishment of cell suspension culture were liquid MS medium with 1.0 mg/L 6-BA and 0.1 mg/L 2,4-D，initial inoculum of 20 g/L，shaking speed of 100 r/min.The suspended particles which were subcultured 3~4 times then cultured on half strength minerals of solid MS medium with 1.0 mg/L 6-BA and 0.1 mg/L 1-naphthylacetic acid (NAA) for inducing regeneration.The buds came out in 3 weeks and later complete plants would be obtained.The suspension culture system had important application value in improving study which taking Torenia fournieri L. as a model plant.

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