Effect of Low Temperture Stress on Inclusions Change and Cell Ultrastructure of Jerusalem artichoke Tuber(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Effect of Low Temperture Stress on Inclusions Change and Cell Ultrastructure of Jerusalem artichoke Tuber

Author(s):: WANG Li-na¹; 2; YIN Kui-de¹; JIN Xun²; LI Liang²; REN Cui-mei²; YU Ji-dong²; SONG Qun-yan¹; 1.Heilongjiang Bayi Agricultural University，Daqing，Heilongjiang 163319；
2.Daqing Branch，Heilongjiang Academy of Agricultural Sciences，Daqing，Heilongjiang 163316

Keywords:: Jerusalem artichoke; physiological index; ultrastructure; cold stress

Abstract:: Using ‘Qingyu No.1’ and ‘Shengyu’ as materials,the content of total sugar，reducing sugar，soluble sugar and starch of two varieties Jerusalem artichoke tuber under the nature low temperture stress were determined.Meanwhile，tuber cells ultrastructure under cold stress by scanning transmission electron microscopy were also observed.The results showed that ‘Qingyu No.1’ had total sugar increasing，reducing sugar increasing，soluble sugar increasing，starch reducing trend.Otherwise，‘Shenyu’ had total sugar reducing，reducing sugar small increasing，soluble sugar reducing，starch more reducing trend by statistical analysis.The results of cells ultrastructure observation showed that cold resistance tuber ‘Qingyu No.1’ was vacuole crushing，the number of mitochondria increasing，deformation，disintegration under -20℃,but showed by non-cold resistance tuber ‘Shenyu’ under 5℃,this order was identical with plants cytop cell defense phenomenon cope with cold environment condition.

[1]刘祖祺,张石诚.植物抗性生理学[M].北京:农业出版社，1994:13-15.

[2]何若韬,王光洁.植物寒冷驯化机理[J].植物生理生化进展,1987(5):17-29.

[3]Li P H,Chen H H.Plant cold hardiness and freezing stresses[M].New York:Academic Press,1982:5-22.

[4]潘红丽,王殿奎.能源植物菊芋的品种鉴定和筛选[J].黑龙江农业科学,2008(4):79-80.

[5]王学奎.植物生理生化实验原理和技术[M].北京:高等教育出版社,2006:174-176.

[6]李雯,邵远志,陈维信.淀粉酶活性测定方法的改进[J].植物生理学通讯,2005,41(5):655-656.

[7]张景云,白雅梅,于萌,等.盐胁迫对二倍体马铃薯叶超微结构的影响[J].东北农业大学学报,2010,41(8):7-10.

[8]简令成,孙龙华.抗冻植物避免细胞内结冰机制的探讨-液泡内吞作用和水外排渠道的发现.植物学集刊[M].北京:科学出版社,1983:107-113.

[9]周蕴薇.翠南报春叶片细胞超微结构对低温的适应性变化[J].园艺学报,2006,33(6)：1361-1364.

[10]纪忠雄.柑橘抗寒性的生理生化指标[J].园艺学报,1983,10(4):239-244.

[11]扬阿明,沈征言.低温锻炼提高黄瓜幼苗耐寒性效应[J].园艺学报,1992,19(1):61-66.

[12]姜卫兵,王业遴,马凯.渗透保护物质在无花果抗寒性发育中的作用[J].园艺学报,1992,19(4):371-372.

[13]朱月林,曹寿椿.不结球白菜抗冻性鉴定及其相关性状的研究[J].园艺学报,1989,16(2):127-133.

[14]刘祖祺,张石城.植物抗性生理学[M].北京:中国农业出版社,1994.

[15]周蕴薇.低温锻炼和低温胁迫期间翠南报春叶肉细胞超微结构的适应变化[J].植物生理学通讯,2007,43(2):255-258.

（该文作者还有宋群雁，工作单位为黑龙江八一农垦大学。）

Last Update: 2014-08-25