Preliminary Study on the Screening of Germplasm Resources and the in Vitro Propagation for Gentiana rigescens(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2013年08期

Page:

168-172

Research Field:

Publishing date:

Info

Title:

Preliminary Study on the Screening of Germplasm Resources and the in Vitro Propagation for Gentiana rigescens

Author(s):

ZHAO Zhi-lian¹; 2; LI Hai-feng¹; ZHANG De-quan¹; DUAN Bao-zhong¹

1.College of Pharmacy and Chemistry，Dali University，Dali，Yunnan 671000；

2.Dali Agriculture School of Yunnan Province，Dali，Yannan 671003

Keywords:

Gentiana rigescens Franch; germplasm resources; gentiopicroside; in vitro propagation; plant regeneration

PACS:

Q 945

DOI:

-

Abstract:

Taking different population and the different individuals within the same population of Gentiana rigescens Franch as materials，the gentiopicroside content in the rhizome of Gentiana rigescens Franch was determined by HPLC method，then the high quality germplasm resources of which the gentiopicroside content was greater than or equal to 8.0% were selected as the explants，and the effects of culture medium and culture temperature on the in vitro propagation and plant regeneration of Gentiana rigescens Franch were explored.The results showed that the gentiopicroside content in rhizome of Gentiana rigescens Franch from different populations of Dali Cangshan reached significant or extremely significant level.Among them，in Gantong population，Zhonghe population，Wanqiao population，and Hudiequan population，the gentiopicroside contents were high，and the content reached more than 8.0%.In Shangguan population，the gentiopicroside content was the lowest (5.849±0.0149%).Within the same population，the gentiopicroside contents of individual plant in rhizome of G.rigescens had small difference.The difference in Xizhou population reached significant level (P<0.05)，in Zhonghe population reached extremely significant level (P<0.01)，while in other populations，the gentiopicroside contents had no significant difference.The most suitable culture medium for stem-tip growth-point in vitro propagation and plant regeneration of Gentiana rigescens Franch was 1/2MS，and the induction frequency of plant regeneration could reach 80.00%，adventitious buds height (5.6±0.2) cm.The optimal culture condition was variable temperature culture at 20/25℃，and the regenerated plant induction frequency could reach 87.78%，with adventitious buds height (7.5±0.2) cm.

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