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《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2013年08期

Page:

116-118

Research Field:

Publishing date:

Info

Title:

Growth Curves of Oleaginous Yeast in High Density Liquid Medium Determined by Spectrophotometry

Author(s):

MA Yong; TU Ya; CHEN Xiu-li; MEN Zhong-hua

Department of Biological Sciences and Technology，Baotou Teachers College，Baotou，Inner Mongolia 014030

Keywords:

oleaginous yeast; spectrophotometry; growth curve; regressive equation

PACS:

Q 943.1

DOI:

-

Abstract:

The oleaginous yeast OD₆₀₀ was determined through the spectrophotometer in different growth time which was in the high density liquid medium，and the high density liquid medium was diluted in different dilution which was in different growth time.Using the method of establishing set of regression equation of OD₆₀₀，yeast Y1 growth curve was drawn using the OD₆₀₀ values after the reunification.The results showed that the growth curve can accurately reflect the cell growth regular.Bacterial cells of yeast Y1 occurred four stages under suitable growth conditions，lag phase，0~4 hours；logarithmic phase，4~24 hours；stationary phase：24~68 hours；decline phase：after 68 hours.This research aimed to provide research solutions to similar problems in detail and important information for related researchers.

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Memo

Memo:

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Common functions

Last Update: 2014-08-25