In vitro Conservation Technique of Germplasm in Zingiber officinale Rosc.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2013年08期

Page:

112-116

Research Field:

Publishing date:

Info

Title:

In vitro Conservation Technique of Germplasm in Zingiber officinale Rosc.

Author(s):

WEI Kun-hua¹; 2; LI Lin-xuan¹; MIAO Jian-hua¹; GAO Shan-lin²

1.Guangxi Botanical Garden of Medicinal Plants,Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement，Nanning，Guangxi 530023；

2.Department of Genetics and Breeding，China Pharmaceutical University，Nanjing，Jiangsu 211198

Keywords:

Zingiber officinale Rosc.; germplasm resource; in vitro conservation; growth recovery; genetic stability

PACS:

S 632.502

DOI:

-

Abstract:

To establish the optimal in vitro conservation technology system of Zingiber officinale Rosc.germplasm，the effects of phytohormone，osmotic pressure and temperature on it were tested，and the growth recovery and genetic stability were evaluated after 300 days of in vitro conservation.The results showed that the best conservation conditionwas MS basal medium containing 6 g/L sucrose，4.0 g/L agar powder and 10 g/L mannite supplemented with 2.0 mg/L CCC，2.0 mg/L PP₃₃₃ and 2.0 mg/L ABA，in an illuminated chambr under 14~16 h photoperiod of 1 500 lx light intensity at 20℃，more than 50% conservation material survived after 300 days，most of them could grew well on propagation medium，and the chromosome number maintained stably.

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