Comparative Study on Different Extraction Methods of Total RNA from Stem of Manihot esculenta Crantz(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Comparative Study on Different Extraction Methods of Total RNA from Stem of Manihot esculenta Crantz

Author(s):: ZENG Wen-dan¹; 2; LUO Xing-lu¹; YUAN Sheng-yong¹; YANG Xin¹; GUO Ya-jing¹; 2; 1.College of Agriculture，Guangxi University，Nanning，Guangxi 530005；
2.Guangxi Crop Genetic Improvement and Biotechnology Lab，Nanning，Guangxi 530007

Keywords:: Manihot esculenta Crantz; total of RNA; different extraction methods; extraction effect

Abstract:: Taking Manihot esculenta Crantz as material，the effects of four different extraction methods (Guanidinium isothiocyanate method，Thermal Borate method，Trizol and TransZol Plant) of total RNA from stems of Manihot esculenta Crantz were studied,in order to obtain the efficient method for RNA extraction.The results showed that total RNA of Manihot esculenta Crantz can’t be extracted using the Thermal Borate method，and total cassava stems RNA can be extracted effectively using the other three methods.The time (only 1.5 h) and quality of RNA extracted by Guanidinium isothiocyanate method were the best，which was a kind of simple and efficient method suitable for the extraction of total RNA from the stems of Manihot esculenta Crantz.

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Last Update: 2014-08-25