Study on Optimization of Factors Related to Regeneration of the Tomato(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2013年08期

Page:

93-96

Research Field:

Publishing date:

Info

Title:

Study on Optimization of Factors Related to Regeneration of the Tomato

Author(s):

YIN Li-hui¹; HOU Lei-ping¹; WANG Ting-ting¹; GAO Xing-ying¹; ZHANG Yan-liang²; LI Mei-lan¹

1.College of Horticulture,Shanxi Agricultural University，Taigu，Shanxi 030801；

2.Shanxi Province Seeds Master Station，Taiyuan，Shanxi 030006

Keywords:

tomato; cotyledon; regeneration; elongation

PACS:

S 641.2

DOI:

-

Abstract:

Taking Lycopersicum esculentum ‘Zhongshu No.4’ as test material，the effects of different seedling ages and different organic additives on callus induction and bud differentiation were studied.The best medium for adventitious shoot elongation and rooting were screened.The results showed that the regeneration rate was high when the seedling was in the age of 8~9 d；regeneration from the cotyledon cultured in MS with 0.07% EMS as the organic matter+ZT 2.0 mg/L+IAA 0.1 mg/L was better than in MS with N+N organic matter；the best medium for adventitious shoot elongation was MS+ZT 1.0 mg/L+GA 1.0 mg/L，while for rooting was 1/2MS+IAA 0.3 mg/L.This research facilitated the modification of tomato traits by genetic engineering technology in the future.

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