Study on Isolation and Culture of the Mesophyll Protoplasts from Abutilon avicennae(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2013年07期

Page:

170-172

Research Field:

Publishing date:

Info

Title:

Study on Isolation and Culture of the Mesophyll Protoplasts from Abutilon avicennae

Author(s):

XU Qin¹; WANG Xiao-jun¹; WANG Hui¹; 2; LIU Min¹; HAO Xiu-ying¹

1.Xinjiang Institute of Physics and Chemistry，Chinese Academy of Sciences，Urumqi，Xinjiang 830011；

2.Graduate School，Chinese Academy of Sciences，Beijing 100039；

3.Institute of Microbiology，Xinjiang Academy of Agricultural Sciences，Urumqi，Xinjang 830091

Keywords:

Abutilon avicennae; protoplast; isolation

PACS:

S 563.6

DOI:

-

Abstract:

Taking the leaves and seeds of Abutilon avicennae as materials，the factors affecting the isolation mesophyll protoplasts from Abutilon avicennae were studied.The results showed that the best experiment materials were the leaves from the sterile seeding of Abutilon avicennae；if digested the leaves with combination of the enzyme solution 0.5% cellulase+0.3% macerozyme for 12~14 hours，more better protoplasts will be gotten.In the DPD liquid medium，the cells grew better.

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