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Study on Deproteinization of Exopolysaccharide from Poria cocos Mycelia(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年07期
Page:
151-153
Research Field:
Publishing date:

Info

Title:
Study on Deproteinization of Exopolysaccharide from Poria cocos Mycelia
Author(s):
HAN Yong; WANG Xian-zhi; ZHANG Hong
Shanxi Pharmaceutical Vocational College，Taiyuan，Shanxi 030031
Keywords:
Poria cocos mycelia; exopolysaccharide; deproteinization; enzyme-Sevag method
PACS:
S 567.3+2
DOI:
-
Abstract:
Taking the rate of removed protein and rate of lost polysaccharide as indexes,the effect of sevag method，trichloroacetic acid method，enzyme method，enzyme-Sevag method on deproteinization of exopolysaccharide from Poria cocos mycelia were studied.The results showed that the enzyme-Sevag method was the best method of removing protein from the exopolysaccharide of Poria cocos mycelia.The optimal conditions of deproteinization were as follows：1.5% enzyme，temperature at 50℃，pH value 6.5，deproteinization time 2 h，Sevag deproteinization 3 times treatment.The rate of removed protein and the rate of lost polysaccharide were 87.43% and 10.97%，respectively.

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Last Update: 2014-08-24