|Table of Contents|

Genetic Identification of Celosia Mutant and Select Lines Using Sequence-related Amplified Polymorphism，SRAP(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年07期
Page:
128-130
Research Field:
Publishing date:

Info

Title:
Genetic Identification of Celosia Mutant and Select Lines Using Sequence-related Amplified Polymorphism，SRAP
Author(s):
LIU Xiao-fei; LIAO Fei-xiong; LIU Xiao-rong; LIU Jin-mei; YU Bo
Guangdong Provincial Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization，Institute of Floricultural Research，Guangdong Academy of Agricultural Sciences，Guangzhou，Guangdong 510640
Keywords:
Celosia cristata; SRAP; fingerprint
PACS:
S 681.3
DOI:
-
Abstract:
Taking Celosia cristata ‘Zaoshuijiguan’ and its three selected lines from the mutants，two cultivars from C.plumos ‘Indian’ as materials,the genetic diversity using Sequence-related Amplified Polymorphism (SRAP) were studied.The results showed that one primer pairs me6/g38 from 64 primer pairs amplified stable polymorphism among the 6 varieties with 7 amplifications.All the 6 lines tested could be identified each other by the fingerprint map constituted using the amplifications from me6/g38.The results of Cluster analysis (UPGMA) based on this fingerprint map showed ‘Zaoshuijiguan’ and three mutation lines，which were carried by satellite and cultured for generation，could clustered into a group at cofficient 0.52，while two Indian lines were clustered into a groups at cofficient 0.84.It indicated that SRAP would be a potential molecular marker tool in germplasm identification and genetic diversity analysis of celosia.

References:

[1]陈俊愉,程绪珂.中国花经[M].上海：上海文化出版社,1990:265.

[2]Hase K，Basnet P，Kadota S，et al.Immuno stimulating activity of Celosian，an antihepatotaoxic polysaccharide isolated from Celosia argentea[J].Planta Med,1997,63:216-219.

[3]Hayakawa Y，Fujii H，Hase K，et al.Antimetastatic and immunomodulating properties of the water extract from Celosia argentea seeds[J].BiolPharm Bull,1998,21:1154-1159.

[4]Tadao A.Amino acids[M].Japan：The Japan essential amino acids association.Incorporated,1987：13-18.

[5]姚悦梅,毛忠良,潘永飞,等.播种期对不同品种鸡冠花观赏性及结实性的影响[J].江西农业学报,2009,21(10):54-56.

[6]姜一凡,徐维杰,廖飞雄,等.花卉空间诱变效应及育种研究进展[J].中国农学通报,2007,23(8):339-342.

[7]夏瑾华.新型鸡冠花的品种选育及其遗传学研究[D].武汉:湖北大学,2004.

[8]张继业，钟仙龙,李锡珍,等.60Co-r射线辐射鸡冠花种子对其发芽率及幼苗生长的影响[J].安徽农业科学,2005,33(4):620-720.

[9]张孟锦,廖飞雄,王碧青,等.卫星搭载处理对鸡冠花SPl代种子萌发及生长发育的影响[J].广东农业科学,2006(4):37-39.

[10]Li G，Quiros C F.Sequence-related amplified polymorpgism (SRAP) a new marker system based on a simple PCR reaction：its application to mapping and gene tagging in Brassica[J].Theor Appl Genet,2001,103:455-461.

[11]王燕,龚义勤,赵统敏,等.番茄SRAP-PCR体系优化与品种分子鉴定[J].南京农业大学学报,2007,30(1):23-29.

[12]任羽,王得元,张银东.辣椒SRAP-PCR反应体系的建立与优化[J].分子植物育种,2004,2(5):689-693.

[13]吴洁,谭文芳,何俊蓉.甘薯SRAP连锁图构建淀粉含量QTL检测[J].分子植物育种,2005,3(6):841-845.

[14]李严,张庆春.西瓜杂交种遗传多样性的SRAP标记分析[J].园艺学报,2005,32(4):643-647.

[15]Feng N，Xue Q，Guo Q H，et al.Genetic diversity and population structure of Celosia argentea and related speciesrevealed by SRAP[J].Biochem Genet,2009,47:521-532.

[16]姜一凡.卫星搭载鸡冠花后代的生物学效应及株系筛选[D].南昌:江西农业大学,2008.

[17]Liu L，Guo W，Zhu X，et al.Inheritance and fine mapping of fertility restoration for cytoplasmic male sterility in Gossypium hirsutum?L[J].Theor Appl Genet,2003,106:461-469.

[18]Sneath P H A，Sokal R R.Numerical taxonomy[C].Freeman,San Francisco,CA,1973.


Memo

Memo:
-
Last Update: 2014-08-24