Study on the Factors of Onion Callus and Embryonic Callus Induction(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2013年07期

Page:

125-127

Research Field:

Publishing date:

Info

Title:

Study on the Factors of Onion Callus and Embryonic Callus Induction

Author(s):

DAI Yue¹; WU Xiong²; LIAN Mei-lan¹; HUO Yu-meng²; MIAO Jun²; PIAO Xuan-chun¹

1.College of Agriculture，Yanbian University，Yanji，Jilin 133002;

2.Institute of Vegetable Research,Shandong Academy of Agricultural Sciences,Jinan,Shandong 250100

Keywords:

onion (Allium cepa L.); callus; embryogenic callus; tissue culture

PACS:

S 633.203.6

DOI:

-

Abstract:

Taking the male sterility line seeds of onion (Allium cepa L.) as explants，the effects of sucrose concentration，light and sampling time on callus tissues and embryogenic callus tissues induction were studied，in order to optimize the conditions of embryogenic callus formation，and provide a good receptor transgenic and lay the foundation for the establishment of high frequency regeneration system.The results showed that 40 g/L sucrose and light culture were the most suitable for callus induction；50 g/L sucrose and dark culture were the most suitable for embryogenic callus induction，and the optimum sampling time was the 45th day.

References:

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