Study on Tissue Culture and Plant Regeneration in Castorplant Regeneration of Xanthoceras sorbifolia Bunge.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Tissue Culture and Plant Regeneration in Castorplant Regeneration of Xanthoceras sorbifolia Bunge.

Author(s):: SONG Qun-yan¹; WANG Li-yan¹; JIAO Hong-shuang²; WANG Li-na¹; YIN Kui-de¹; 1.Heilongjiang Bayi Agricultural University，Daqing，Heilongjiang 163319；
2.Farming Branch of Kunlun Group，Daqing Oil Field Company，Daqing，Heilongjiang 163458

Abstract:: Taking the tender leaves of Xanthoceras sorbifolia as explants，the effect of species and concentrations of plant growth regulator on tissue culture and plant regeneration of Xanthoceras sorbifolia were studied，after the following four steps：adventitious shoot induction，induction and proliferation and differentiation，root induction，and root growth，an effective regeneration system was established for Xanthoceras sorbifolia.The results showed that the optimal medium for callus induction was DKW+6-BA 0.5 mg/L+NAA 0.5 mg/L+2,4-D 1.0 mg/L；for callus differentiation and proliferation the optimal medium was DKW+6-BA 0.5 mg/L+NAA 0.5 mg/L+KT 1.0 mg/L；for elongating DKW+6-BA 0.2 mg/L+IBA 0.5 mg/L，and for rooting，1/2MS +IBA 0.5 mg/L；the ratio of rooting was 81%，and that of survival was more than 90% when small intact plants were transplanted.The establishment of this regeneration system had laid a foundation for rapid propagation and genetic transformation of Xanthoceras sorbifolia.

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Last Update: 2014-08-24