7 mushroom varieties (strains) were introduced into Yinjiang(‘241-4’，‘135’，‘937’，‘939’，‘Shenxiang No.4’,‘Xiangxuan No.2’,‘Mixiang No.32’)，and all varieties (strains) of Lentinus edodes mycelium growth，fruiting characteristics and yield were studied.The results showed that mushroom strains ‘241-4’,‘135’，and ‘939’ had better performance on mycelial growth rate，hypha growth，fruiting growth characters and yield.The mushroom yield had significant difference or extremely significant difference between the three strains with other tested strains respectively.Biological conversion rates of the three strains were all more than 65%，so they were suitable for popularization and application in Guizhou mountain area.