Study on Artificial Cultivation Optimization of Solid Culture Medium of Cordyceps militaris (PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Artificial Cultivation Optimization of Solid Culture Medium of Cordyceps militaris

Author(s):: QIN Xiu-li; XING Li; YIN Rui; Jilin Agriculture Science and Technology College，Jilin，Jilin 132101

Keywords:: Cordyceps militaris; solid culture; nutrient solution; orthogonal test

Abstract:: Artificial cultivation of solid culture medium of Cordyceps militaris were optimized in this experiment.The results showed that the C.militaris could be cultivated in many types of solid culture medium，the growth rate and density of the hyphae were the highest in a single solid culture medium with rice as the solid substrate，but adding 10%～15% wheat bran in rice solid culture medium，the growth rate and density of the hyphae were better than the single matrix of rice；the suitable nutrient solution was determined by contrast test and L₉(3⁴) orthogonal test，the composition was sucrose 2.0%，yeast extract 1.5%，MgSO₄ 0.10%，KH₂PO₄0.10%；the suitable solid culture medium of the C.militaris sinensis was determined by L₉(3³) orthogonal test，the composition was rice 85%，wheat bran 15%，with 60 g substance in 500 mL bottle,pH 5.5~6.5，the ratio of solid and water (solid matrix/nutrient solution) was 1∶1.5.

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Last Update: 2014-08-24