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Identification and Phylogenetic Tree Analysis of Tomato Gray Mould Pathogen(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年06期
Page:
127-131
Research Field:
Publishing date:

Info

Title:
Identification and Phylogenetic Tree Analysis of Tomato Gray Mould Pathogen
Author(s):
WANG Rui-hu1; GUAN Xin1; CHEN Xiu-ling1; WANG Ao-xue1; 2
1.College of Horticulture，Northeast Agricultural University，Harbin，Heilongjiang 150030；
2.College of Life Sciences，Northeast Agricultural University，Harbin，Heilongjiang 150030
Keywords:
tomato gray mould; Botrytis cinerea; strain isolation; identification
PACS:
S 436.412.1+3
DOI:
-
Abstract:
Strain t08016b，which morphological characteristics was very similar to the Botrytis spp.，was isolated and purified from infected tomato fruits with typical symptoms of grey mold rot.The sequences of 18S rDNA and ITS1-5.8S-ITS2 rDNA of strain t08016b were specifically amplified and sequenced using universal primer pairs NS1/NS8 and ITS4/ITS5.The results showed that the 18S sequence of t08016b had 99.94% similarity with fuckeliana fuckeliana (EF110887.1) and the ITS1-5.8S-ITS2 rDNA sequence had 100% similarity with Botryotinia fuckeliana (AB444949.1).The results of sequence alignment and neighbor-joining tree of 18S and ITS1-5.8S-ITS2 rDNA showed that t08016b was one strain of Botryotinia fuckeliana.The incidence of tomato grey mould could up to 97% after infected by strain t08016b which indicated that t08016b possess strong pathogenicity.Comprehensive morphological identification and molecular identification and pathogenicity analysis，indicated that strain t08016b was a Botrytis cinerea strain with strong pathogenicity.

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Last Update: 2014-08-24