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Comparison and Analysis of Total RNA Extraction Methods from Populus simonii×P.nigra Pollen(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年06期
Page:
113-116
Research Field:
Publishing date:

Info

Title:
Comparison and Analysis of Total RNA Extraction Methods from Populus simonii×P.nigra Pollen
Author(s):
YUAN Hong-mei1; 2; ZHAO Li-juan3; GUO Wen-dong4; WU Jian-zhong2; ZHANG Li-guo2; GUAN Feng-zhi2
1.State Key Laboratory of Tree Genetics and Breeding，Northeast Forestry University，Harbin，Heilongjiang 150040；
2.Institute of Industrial Crops,Heilongjiang Academy of Agricultural Sciences，Harbin，Heilongjiang 150086；
3.Institute of Crop Breeding,Heilongjiang Academy of Agricultural Sciences，Harbin，Heilongjiang 150086;
4.Institute of Nature and Ecology,Heilongjiang Academy of Sciences，Harbin，Heilongjiang 150040
Keywords:
Populus simonii×P.nigra; pollen; RNA extraction; RT-PCR
PACS:
S 792.11
DOI:
-
Abstract:
Taking the pollen of Populus simonii×P.nigra as material，four kinds of total RNA extraction methods (traditional CTAB method，RNAout kit，improved CTAB method and SDS method) were compared using agarose gel electrophoresis，spectrophotometry and RT-PCR method.The results showed that SDS method was suitable fortotal RNA with high purity and good integrity extraction from Populus simonii×P.nigra pollen.It had also the advantage of simplicity，less operation time and low cost.It was an ideal method for total RNA extraction from Populus simonii×P.nigra pollen.

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Last Update: 2014-08-23