Establishment of Eleocharistuberosa System of Open Tissue Culture in Hezhou(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Establishment of Eleocharistuberosa System of Open Tissue Culture in Hezhou

Author(s):: WU Gui-rong; QU Fen-xia; YU Bing-feng; Province Key Construction Laboratory of Characteristic Resources Research and Development of Guangxi，Hezhou，Guangxi 542800

Abstract:: The ‘Heti No.1’of Eleocharis tuberosa in Hezhou was used as explants，the effects of resist agent type and concentration，the concentration of sucrose，organic ingredients and effects of culture ways in Hezhou open tissue culture were studied.The results showed that hypochlorite sodium concentration for 1.0%，pollution rate was minimum，for 23.3%，average induced rate up to 79.0%；sucrose concentration for 2.0%，the effect was better，pollution rate for 23.0%；organic components removal ammonia acid was pollution minimum，for 11.0%，induced rate up 80.7%；in vaccination material in training in the culturing room，pollution rate were low and induced rate high，respectively for 14.0% and 87.7%，but if glass greenhouse of environment conditions was improved，thus reduced pollution rate，was one of the effective ways to open tissue culture.

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Last Update: 2014-08-23