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Study on Induction of Embryogenic Tissue of Platycladus orientalis (PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年06期
Page:
108-110
Research Field:
Publishing date:

Info

Title:
Study on Induction of Embryogenic Tissue of Platycladus orientalis
Author(s):
CAO Ya-qiong; ZHANG Cun-xu
College of Forestry，Northwest Agricultural and Forestry University,Yangling，Shaanxi 712100
Keywords:
Platycladus orientalis; embryogenic callus; immature zygotic embryo; radicle; initiation factors
PACS:
S 791.38
DOI:
-
Abstract:
Taking the immature zygotic embryo and radicle of Platycladus orientalis as test materials，the effects of basic medium，different explants，plant hormones，zygotic embryo developmental stage in callus induction were studied.The results showed that immature zygotic embryo and radicle could induce on optimal medium.The optimal combination of medium for immature zygotic embryo was DCR+2.0 mg/L 2,4-D+0.2 mg/L 6-BA+0.5 g/L glutamine，the inducing rate was 96.67％.The optimal combination of medium for radicle was DCR+1.0 mg/L 2,4-D+0.1 mg/L 6-BA+0.5 g/L glutamine，the inducing rate was 93.33％.The best period of immature zygotic embryo sampling was around July 3.

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Last Update: 2014-08-23