Study on Tissue Culture and Rapid Propagation of Dendrobium fimbriatum Hook.var. oculatum.Hook(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Tissue Culture and Rapid Propagation of Dendrobium fimbriatum Hook.var. oculatum.Hook

Author(s):: LI Cui; ZHANG Zhan-jiang; WEI Ying; LI Lin-xuan; LING Zheng-zhu; WEI Kun-hua; Guangxi Botanical Garden of Medicinal Platn，Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement，Nanning，Guangxi 530023

Keywords:: Dendrobium fimbriatum Hook.var. oculatum. Hook.; tissue culture; protocorm-liked body; rooting

Abstract:: Taking the seeds of Dendrobium fimbriatum Hook.var. oculatum.Hook.as materials，the tissue culture and rapid propagation in vitro of it were studied，in order to screen the suitable medium in every stage.The results showed that the best medium for protocorm-liked body induction was MS+GA 0.1 mg/L+6-BA 1.5 mg/L，with the inductivity 85%.The best bud differentiation and proliferation medium was MS+KT 0.5 mg/L+6-BA 2.5 mg/L+NAA 1.0 mg/L，with the differentiation rate 90%.The best medium for rooting was MS+AC 0.5 mg/L+NAA 1.0 mg/L，with the rooting rate 100%.

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Last Update: 2014-08-23